Abstract
Real-time PCR provides a fast, reliable, and cost-effective method for detecting the presence or absence of Petri disease fungi in grapevines. The primer pairs, Pmo1f + Pmo2r, and Pac1f + Pac2r, were designed for species and genus-specific amplification of Phaeomoniella chlamydospora and Phaeoacremonium spp. respectively, using real-time PCR with SYBR® Green. The primers were specific and showed no primer-primer dimers until after 35 cycles. Pa. chlamydospora was detected in roots, shoots, and young trunks of drill-inoculated vines. Phaeoacremonium was detected in trunk cross-sections of naturally infected vines from which Phaeoacremonium aleophilum had been isolated. The protocol presented here can be adapted to provide a reliable detection system for research and industry.
Original language | English (US) |
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Pages (from-to) | 403-410 |
Number of pages | 8 |
Journal | Phytopathologia Mediterranea |
Volume | 43 |
Issue number | 3 |
State | Published - 2004 |
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science
- Horticulture