Quantitative analysis of bile acid with UHPLC-MS/MS

Yuan Tian, Jingwei Cai, Erik L. Allman, Philip B. Smith, Andrew D. Patterson

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Quantitative analysis of bile acid with UHPLC-MS/MS'. Together they form a unique fingerprint.

Cite this