Quantitative analysis of bile acid with UHPLC-MS/MS

Yuan Tian; Jingwei Cai; Erik L. Allman; Philip B. Smith; Andrew D. Patterson

doi:10.1007/978-1-0716-0849-4_15

Quantitative analysis of bile acid with UHPLC-MS/MS

Yuan Tian, Jingwei Cai, Erik L. Allman, Philip B. Smith, Andrew D. Patterson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Scopus citations

Abstract

Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	291-300
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-0849-4_15
State	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2194
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-0849-4_15

Cite this

@inbook{bf87b818ec4046e6a0ec2a01ba64ec5c,

title = "Quantitative analysis of bile acid with UHPLC-MS/MS",

abstract = "Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.",

author = "Yuan Tian and Jingwei Cai and Allman, {Erik L.} and Smith, {Philip B.} and Patterson, {Andrew D.}",

note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

doi = "10.1007/978-1-0716-0849-4_15",

language = "English (US)",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "291--300",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Quantitative analysis of bile acid with UHPLC-MS/MS

AU - Tian, Yuan

AU - Cai, Jingwei

AU - Allman, Erik L.

AU - Smith, Philip B.

AU - Patterson, Andrew D.

PY - 2021

Y1 - 2021

N2 - Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.

AB - Bile acids are important end products of cholesterol metabolism, having been shown to serve as signaling molecules and intermediates between the host and the gut microbiota. Here we describe a robust and accurate method using ultrahigh-pressure liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) for the quantification of bile acids in stool/cecal and tissue samples.

UR - http://www.scopus.com/inward/record.url?scp=85091051429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85091051429&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-0849-4_15

DO - 10.1007/978-1-0716-0849-4_15

M3 - Chapter

C2 - 32926372

AN - SCOPUS:85091051429

T3 - Methods in Molecular Biology

SP - 291

EP - 300

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Quantitative analysis of bile acid with UHPLC-MS/MS

Abstract

Publication series

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this