Quantitative determination of urea concentrations in cell culture medium

Robert J.X. Zawada; Peggy Kwan; Kellen L. Olszewski; Manuel Llinas; Shu Gui Huang

doi:10.1139/O09-011

Quantitative determination of urea concentrations in cell culture medium

Robert J.X. Zawada, Peggy Kwan, Kellen L. Olszewski, Manuel Llinas, Shu Gui Huang

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50 Scopus citations

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

Original language	English (US)
Pages (from-to)	541-544
Number of pages	4
Journal	Biochemistry and Cell Biology
Volume	87
Issue number	3
DOIs	https://doi.org/10.1139/O09-011
State	Published - Jun 2009

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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10.1139/O09-011

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abstract = "Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.",

author = "Zawada, {Robert J.X.} and Peggy Kwan and Olszewski, {Kellen L.} and Manuel Llinas and Huang, {Shu Gui}",

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AU - Kwan, Peggy

AU - Olszewski, Kellen L.

AU - Llinas, Manuel

AU - Huang, Shu Gui

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AB - Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplatebased, room temperature assay can be applied to determine urea synthesis in cell culture.

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Quantitative determination of urea concentrations in cell culture medium

Abstract

All Science Journal Classification (ASJC) codes

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