Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

B. S. Coates; A. P. Alves; H. Wang; X. Zhou; T. Nowatzki; H. Chen; M. Rangasamy; H. M. Robertson; C. W. Whitfield; K. K. Walden; S. D. Kachman; B. W. French; L. J. Meinke; D. Hawthorne; C. A. Abel; T. W. Sappington; B. D. Siegfried; N. J. Miller

doi:10.1111/imb.12194

Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

B. S. Coates, A. P. Alves, H. Wang, X. Zhou, T. Nowatzki, H. Chen, M. Rangasamy, H. M. Robertson, C. W. Whitfield, K. K. Walden, S. D. Kachman, B. W. French, L. J. Meinke, D. Hawthorne, C. A. Abel, T. W. Sappington, B. D. Siegfried, N. J. Miller

College of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

Original language	English (US)
Pages (from-to)	1-15
Number of pages	15
Journal	Insect Molecular Biology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1111/imb.12194
State	Published - Feb 1 2016

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics
Insect Science

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Coates, B. S., Alves, A. P., Wang, H., Zhou, X., Nowatzki, T., Chen, H., Rangasamy, M., Robertson, H. M., Whitfield, C. W., Walden, K. K., Kachman, S. D., French, B. W., Meinke, L. J., Hawthorne, D., Abel, C. A., Sappington, T. W., Siegfried, B. D., & Miller, N. J. (2016). Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera. Insect Molecular Biology, 25(1), 1-15. https://doi.org/10.1111/imb.12194

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title = "Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera",

abstract = "The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.",

author = "Coates, {B. S.} and Alves, {A. P.} and H. Wang and X. Zhou and T. Nowatzki and H. Chen and M. Rangasamy and Robertson, {H. M.} and Whitfield, {C. W.} and Walden, {K. K.} and Kachman, {S. D.} and French, {B. W.} and Meinke, {L. J.} and D. Hawthorne and Abel, {C. A.} and Sappington, {T. W.} and Siegfried, {B. D.} and Miller, {N. J.}",

note = "Publisher Copyright: {\textcopyright} Published 2015. This article is a U.S. Government work and is in the public domain in the USA.",

year = "2016",

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Coates, BS, Alves, AP, Wang, H, Zhou, X, Nowatzki, T, Chen, H, Rangasamy, M, Robertson, HM, Whitfield, CW, Walden, KK, Kachman, SD, French, BW, Meinke, LJ, Hawthorne, D, Abel, CA, Sappington, TW, Siegfried, BD & Miller, NJ 2016, 'Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera', Insect Molecular Biology, vol. 25, no. 1, pp. 1-15. https://doi.org/10.1111/imb.12194

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T1 - Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

AU - Coates, B. S.

AU - Alves, A. P.

AU - Wang, H.

AU - Zhou, X.

AU - Nowatzki, T.

AU - Chen, H.

AU - Rangasamy, M.

AU - Robertson, H. M.

AU - Whitfield, C. W.

AU - Walden, K. K.

AU - Kachman, S. D.

AU - French, B. W.

AU - Meinke, L. J.

AU - Hawthorne, D.

AU - Abel, C. A.

AU - Sappington, T. W.

AU - Siegfried, B. D.

AU - Miller, N. J.

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N2 - The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

AB - The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

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Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

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