Quorum Sensing Facilitates Interpopulation Signaling by Vibrio fischeri within the Light Organ of Euprymna scolopes

Taylor A. Yount, Andrew N. Murtha, Andrew G. Cecere, Tim I. Miyashiro

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Quorum sensing is an intercellular signaling mechanism that enables bacterial cells to coordinate population-level behaviors. How quorum sensing functions in natural habitats remains poorly understood. Vibrio fischeri is a bacterial symbiont of the Hawaiian bobtail squid Euprymna scolopes and depends on LuxI/LuxR quorum sensing to produce the symbiotic trait of bioluminescence. A previous study demonstrated that animals emit light when co-colonized by a Δlux mutant, which lacks several genes within the lux operon that are necessary for bioluminescence production, and a LuxI mutant, which cannot synthesize the quorum signaling molecule N-3-oxohexanoyl-homoserine lactone. Here, we build upon that observation and show that populations of LuxI feature elevated promoter activity for the lux operon. We find that population structures comprising of Δlux and LuxI are attenuated within the squid, but a wild-type strain enables the LuxI strain type to be maintained in vivo. These experimental results support a model of interpopulation signaling, which provides basic insight into how quorum sensing functions within the natural habitats found within a host.

Original languageEnglish (US)
Article numbere202200061
JournalIsrael Journal of Chemistry
Volume63
Issue number5-6
DOIs
StatePublished - Jun 2023

All Science Journal Classification (ASJC) codes

  • General Chemistry

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