TY - JOUR
T1 - RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger
AU - Yang, Kailin
AU - Moldovan, George Lucian
AU - D'Andrea, Alan D.
PY - 2010/6/18
Y1 - 2010/6/18
N2 - SNM1A is a member of the SNM1 family of nucleases required for cellular processing of interstrand DNA crosslinks (ICLs). Little is known about the molecular function of SNM1A, in terms of its recruitment to ICL lesions or its DNA damage processing activity. Here we show that SNM1A contains a functional PIP box (PCNA-interacting protein box) and a UBZ (ubiquitin binding zinc finger), required for assembly ofSNM1A into nuclear focus. Moreover, RAD18-dependent monoubiquitination of PCNA is required for Mitomycin C and Ultraviolet Light inducible SNM1A nuclear focus assembly. Taken together, our results identify a novel RAD18-PCNA(Ub)-SNM1A pathway required for nuclear focus formation and ICL resistance.
AB - SNM1A is a member of the SNM1 family of nucleases required for cellular processing of interstrand DNA crosslinks (ICLs). Little is known about the molecular function of SNM1A, in terms of its recruitment to ICL lesions or its DNA damage processing activity. Here we show that SNM1A contains a functional PIP box (PCNA-interacting protein box) and a UBZ (ubiquitin binding zinc finger), required for assembly ofSNM1A into nuclear focus. Moreover, RAD18-dependent monoubiquitination of PCNA is required for Mitomycin C and Ultraviolet Light inducible SNM1A nuclear focus assembly. Taken together, our results identify a novel RAD18-PCNA(Ub)-SNM1A pathway required for nuclear focus formation and ICL resistance.
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U2 - 10.1074/jbc.M109.100032
DO - 10.1074/jbc.M109.100032
M3 - Article
C2 - 20385554
AN - SCOPUS:77953534058
SN - 0021-9258
VL - 285
SP - 19085
EP - 19091
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 25
ER -