TY - JOUR
T1 - Radiation hybrid map of the porcine genome comprising 2035 EST loci
AU - Rink, Anette
AU - Eyer, Katie
AU - Roelofs, Benjamin
AU - Priest, Kimberly J.
AU - Sharkey-Brockmeier, Kathleen J.
AU - Lekhong, Sudkamon
AU - Karajusuf, Elif K.
AU - Bang, Jason
AU - Yerle, Martine
AU - Milan, Denis
AU - Liu, Wan Sheng
AU - Beattie, Craig W.
PY - 2006/8
Y1 - 2006/8
N2 - The IMpRH7000-rad radiation hybrid panel was used to map 2035 expressed sequence tags (ESTs) at a minimum LOD score of 4.0. A total of 134 linkage groups covers 57,192 cR or 78% of the predicted size of the porcine and 71% of the human genome, respectively. Approximately 81% (1649) of the porcine ESTs were annotated against the NCBI nonredundant database; 1422 mapped in silico to a location in build 35.1 of the human genome sequence (HGS) and 1185 to a gene and location in build 35.1 HGS. The map revealed 40 major breaks in synteny (1.00e -25 and lower) with the human genome, 37 of which fall within a single chromosome. At this improved level of resolution and coverage, porcine chromosomes (SSC) 2, 5, 6, 7, 12, and 14 remain "gene-rich" and homologous to human chromosomes (HSA) 17, 19, and 22, while SSC 1, 8, 11, and X have been confirmed to correspond to the "gene-deserts" on HSA 18, 4, 13, and X.
AB - The IMpRH7000-rad radiation hybrid panel was used to map 2035 expressed sequence tags (ESTs) at a minimum LOD score of 4.0. A total of 134 linkage groups covers 57,192 cR or 78% of the predicted size of the porcine and 71% of the human genome, respectively. Approximately 81% (1649) of the porcine ESTs were annotated against the NCBI nonredundant database; 1422 mapped in silico to a location in build 35.1 of the human genome sequence (HGS) and 1185 to a gene and location in build 35.1 HGS. The map revealed 40 major breaks in synteny (1.00e -25 and lower) with the human genome, 37 of which fall within a single chromosome. At this improved level of resolution and coverage, porcine chromosomes (SSC) 2, 5, 6, 7, 12, and 14 remain "gene-rich" and homologous to human chromosomes (HSA) 17, 19, and 22, while SSC 1, 8, 11, and X have been confirmed to correspond to the "gene-deserts" on HSA 18, 4, 13, and X.
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U2 - 10.1007/s00335-005-0121-0
DO - 10.1007/s00335-005-0121-0
M3 - Article
C2 - 16897346
AN - SCOPUS:33747164802
SN - 0938-8990
VL - 17
SP - 878
EP - 885
JO - Mammalian Genome
JF - Mammalian Genome
IS - 8
ER -