TY - JOUR
T1 - Randomly amplified polymorphic DNA reveals fine-scale genetic structure in Pissodes strobi (Coleoptera: Curculionidae)
AU - Lewis, K. G.
AU - Ritland, K.
AU - El-Kassaby, Y. A.
AU - McLean, J. A.
AU - Glaubitz, J.
AU - Carlson, J. E.
N1 - Funding Information:
We thank SP Taylor, B Paulard, C Chu, A Applejohn, N Humphreys, D Clark, and E Tomlin for providing the weevils for this study. We thank two anonymous reviewers and the Editor (Jean Turgeon) for comments that greatly improved the manuscript. This study was supported by a Science Council of British Columbia GREAT award and a VanDusen scholarship to KG Lewis; a Green Plan grant to R Alfaro; the Rothwell Research Fund; a Natural Sciences and Engineering Research Council of Canada (NSERC) research grant to JE Carlson; and a NSERC research chair held by K Ritland.
PY - 2001
Y1 - 2001
N2 - To confirm patterns of diversity and differentiation found with isozymes and mitochondrial DNA, we surveyed 10 populations of the white pine weevil, Pissodes strobi (Peck), for randomly amplified polymorphic DNA (RAPD) markers. Four weevil populations were sampled from Sitka spruce, Picea sitchensis (Bong.) Carr (Pinaceae), five from the "interior" spruce of British Columbia [admixtures of white spruce, Picea glauca (Moench) Voss, and Engelmann spruce, Picea engelmanni (Parry)], and one from Jack pine, Pinus banksiana Lamb. (Pinaceae), in Ontario. In each population, 30-60 weevils were assayed with 10 RAPD primers, yielding 74 RAPD markers. Genetic analyses showed that populations from interior spruce and Jack pine formed a distinct complex; as well, Vancouver Island populations formed a distinct group within the Sitka populations. Levels of diversity, both in terms of polymorphic loci and expected heterozygosity, declined from east to west, supporting the contention that P. strobi originated in eastern North America and migrated west, and suggesting that biocontrol methods may be more effective on populations from Sitka spruce, owing to their reduced diversity. These results parallel an earlier isozyme study but, in contrast, the diversity differences and population relationships are demonstrated to be statistically significant, owing to both the much larger number of loci sampled and the attachment of statistical confidence intervals to estimates of diversity and differentiation.
AB - To confirm patterns of diversity and differentiation found with isozymes and mitochondrial DNA, we surveyed 10 populations of the white pine weevil, Pissodes strobi (Peck), for randomly amplified polymorphic DNA (RAPD) markers. Four weevil populations were sampled from Sitka spruce, Picea sitchensis (Bong.) Carr (Pinaceae), five from the "interior" spruce of British Columbia [admixtures of white spruce, Picea glauca (Moench) Voss, and Engelmann spruce, Picea engelmanni (Parry)], and one from Jack pine, Pinus banksiana Lamb. (Pinaceae), in Ontario. In each population, 30-60 weevils were assayed with 10 RAPD primers, yielding 74 RAPD markers. Genetic analyses showed that populations from interior spruce and Jack pine formed a distinct complex; as well, Vancouver Island populations formed a distinct group within the Sitka populations. Levels of diversity, both in terms of polymorphic loci and expected heterozygosity, declined from east to west, supporting the contention that P. strobi originated in eastern North America and migrated west, and suggesting that biocontrol methods may be more effective on populations from Sitka spruce, owing to their reduced diversity. These results parallel an earlier isozyme study but, in contrast, the diversity differences and population relationships are demonstrated to be statistically significant, owing to both the much larger number of loci sampled and the attachment of statistical confidence intervals to estimates of diversity and differentiation.
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U2 - 10.4039/Ent133229-2
DO - 10.4039/Ent133229-2
M3 - Article
AN - SCOPUS:0035049420
SN - 0008-347X
VL - 133
SP - 229
EP - 238
JO - Canadian Entomologist
JF - Canadian Entomologist
IS - 2
ER -