Rapid and convenient method for the assay of aminopropyltransferases

H. Hibasami; A. E. Pegg

doi:10.1042/bj1690709

Rapid and convenient method for the assay of aminopropyltransferases

H. Hibasami, A. E. Pegg

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33 Scopus citations

Abstract

A new method for the assay of aminopropyltransferase activity is described. The method measures the formation of [methyl-¹⁴C] methylthioadenosine from decarboxylated S-adenosyl[methyl-¹⁴C]methionine in the presence of an amine acceptor. When used with extracts from rat ventral prostate, kidney, liver or brain, and with putrescine or spermidine as amines, the method gave results in excellent agreement with those obtained by the much more time-consuming conventional method. It was found that 1,3-diamino-propane and 1,8-diamino-octane were not acceptors for the prostatic enzyme fraction, but 1,5-diaminopentane (cadaverine) was active and 1,9-diaminononane and 1,12-diaminododecane also lead to the production of [methyl-¹⁴C] methylthioadenosine.

Original language	English (US)
Pages (from-to)	709-712
Number of pages	4
Journal	Biochemical Journal
Volume	169
Issue number	3
DOIs	https://doi.org/10.1042/bj1690709
State	Published - 1978

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

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title = "Rapid and convenient method for the assay of aminopropyltransferases",

abstract = "A new method for the assay of aminopropyltransferase activity is described. The method measures the formation of [methyl-14C] methylthioadenosine from decarboxylated S-adenosyl[methyl-14C]methionine in the presence of an amine acceptor. When used with extracts from rat ventral prostate, kidney, liver or brain, and with putrescine or spermidine as amines, the method gave results in excellent agreement with those obtained by the much more time-consuming conventional method. It was found that 1,3-diamino-propane and 1,8-diamino-octane were not acceptors for the prostatic enzyme fraction, but 1,5-diaminopentane (cadaverine) was active and 1,9-diaminononane and 1,12-diaminododecane also lead to the production of [methyl-14C] methylthioadenosine.",

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year = "1978",

doi = "10.1042/bj1690709",

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pages = "709--712",

journal = "Biochemical Journal",

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T1 - Rapid and convenient method for the assay of aminopropyltransferases

AU - Hibasami, H.

AU - Pegg, A. E.

PY - 1978

Y1 - 1978

N2 - A new method for the assay of aminopropyltransferase activity is described. The method measures the formation of [methyl-14C] methylthioadenosine from decarboxylated S-adenosyl[methyl-14C]methionine in the presence of an amine acceptor. When used with extracts from rat ventral prostate, kidney, liver or brain, and with putrescine or spermidine as amines, the method gave results in excellent agreement with those obtained by the much more time-consuming conventional method. It was found that 1,3-diamino-propane and 1,8-diamino-octane were not acceptors for the prostatic enzyme fraction, but 1,5-diaminopentane (cadaverine) was active and 1,9-diaminononane and 1,12-diaminododecane also lead to the production of [methyl-14C] methylthioadenosine.

AB - A new method for the assay of aminopropyltransferase activity is described. The method measures the formation of [methyl-14C] methylthioadenosine from decarboxylated S-adenosyl[methyl-14C]methionine in the presence of an amine acceptor. When used with extracts from rat ventral prostate, kidney, liver or brain, and with putrescine or spermidine as amines, the method gave results in excellent agreement with those obtained by the much more time-consuming conventional method. It was found that 1,3-diamino-propane and 1,8-diamino-octane were not acceptors for the prostatic enzyme fraction, but 1,5-diaminopentane (cadaverine) was active and 1,9-diaminononane and 1,12-diaminododecane also lead to the production of [methyl-14C] methylthioadenosine.

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JF - Biochemical Journal

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Rapid and convenient method for the assay of aminopropyltransferases

Abstract

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