TY - JOUR
T1 - Rapid and efficient purification of Cowpea chlorotic mottle virus by sucrose cushion ultracentrifugation
AU - Ali, Akhtar
AU - Roossinck, Marilyn J.
PY - 2007/4
Y1 - 2007/4
N2 - A simple, economical and efficient method for isolation of Cowpea chlorotic mottle virus (CCMV) particles was developed. Abundant viral particles could be obtained with three steps of 10 min duration each of conventional differential centrifugations and one sucrose cushion ultracentrifugation step of 2 h. Characterization of purified virus assessed by transmission electron microscopy, polyacrylamide gel electrophoresis (PAGE) and Western blot revealed the typical isometric particles of CCMV without any visible contamination or degradation. RNA extracted from purified CCMV particles showed all four RNA components by agarose gel electrophoresis. Both purified virus particles and RNA were biologically active and initiated successful infection upon inoculation to cowpea.
AB - A simple, economical and efficient method for isolation of Cowpea chlorotic mottle virus (CCMV) particles was developed. Abundant viral particles could be obtained with three steps of 10 min duration each of conventional differential centrifugations and one sucrose cushion ultracentrifugation step of 2 h. Characterization of purified virus assessed by transmission electron microscopy, polyacrylamide gel electrophoresis (PAGE) and Western blot revealed the typical isometric particles of CCMV without any visible contamination or degradation. RNA extracted from purified CCMV particles showed all four RNA components by agarose gel electrophoresis. Both purified virus particles and RNA were biologically active and initiated successful infection upon inoculation to cowpea.
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U2 - 10.1016/j.jviromet.2006.11.038
DO - 10.1016/j.jviromet.2006.11.038
M3 - Article
C2 - 17188758
AN - SCOPUS:33847068756
SN - 0166-0934
VL - 141
SP - 84
EP - 86
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -