TY - GEN
T1 - RAPID DETECTION OF NOVEL CORONAVIRUS SARS-COV-2 BY SOLID-STATE NANOPORE
AU - Tang, Zifan
AU - Nouri, Reza
AU - Zhu, Yusheng
AU - Kuchipudi, Suresh
AU - Guan, Weihua
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The COVID-19 pandemic spreads rapidly and globally. To quell the pandemic propagation, rapid and accurate detection of SARS-CoV-2 is urgently needed. Here, we present a nanopore coupled RT-LAMP method for SARSCoV-2 detection. After comparing all information from the nanopore experiment, we develop a method to use the event rate change of the amplicons translocation event to measure the amplification. As a result, our platform can distinguish positive from negative samples in 15 min with around 65 copies/reaction limit of detection and 100% specificity. We believe that the nanopore coupled RT-LAMP platform would provide a sensitive and specific detection for SARS-COV-2.
AB - The COVID-19 pandemic spreads rapidly and globally. To quell the pandemic propagation, rapid and accurate detection of SARS-CoV-2 is urgently needed. Here, we present a nanopore coupled RT-LAMP method for SARSCoV-2 detection. After comparing all information from the nanopore experiment, we develop a method to use the event rate change of the amplicons translocation event to measure the amplification. As a result, our platform can distinguish positive from negative samples in 15 min with around 65 copies/reaction limit of detection and 100% specificity. We believe that the nanopore coupled RT-LAMP platform would provide a sensitive and specific detection for SARS-COV-2.
UR - http://www.scopus.com/inward/record.url?scp=85136961174&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136961174&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136961174
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 85
EP - 86
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -