Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Narasimha V. Hegde; Bhushan M. Jayarao; Chitrita DebRoy

doi:10.1128/JCM.00137-12

Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Narasimha V. Hegde, Bhushan M. Jayarao, Chitrita DebRoy

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 10³ target cells in a bacterial mixture of 10 ⁵ CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

Original language	English (US)
Pages (from-to)	2137-2139
Number of pages	3
Journal	Journal of clinical microbiology
Volume	50
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00137-12
State	Published - Jun 2012

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.00137-12

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abstract = "Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 103 target cells in a bacterial mixture of 10 5 CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.",

author = "Hegde, {Narasimha V.} and Jayarao, {Bhushan M.} and Chitrita DebRoy",

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AU - DebRoy, Chitrita

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AB - Rapid, sensitive, and highly specific flow-cytometric assays were developed for the detection of the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) O groups in ground beef. The analytical sensitivity of the assays was 2 × 103 target cells in a bacterial mixture of 10 5 CFU/ml, and the limit of detection in ground beef was 1 to 10 CFU following 8 h of enrichment. The assays may be utilized for rapid detection of STEC O groups in meat.

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Rapid detection of the top six non-O157 Shiga toxin-producing Escherichia coli O groups in ground beef by flow cytometry

Abstract

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