Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS

Daniel J. Weston; Diane E. Grotz; Kathleen A. Cox; Jose Castro-Perez; Steve Preece; Richard Tyldesley; Hilary Major; Daniel J. Hayes; Joseph D. Cuiffi; Stephen J. Fonash

Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS

Daniel J. Weston, Diane E. Grotz, Kathleen A. Cox, Jose Castro-Perez, Steve Preece, Richard Tyldesley, Hilary Major, Daniel J. Hayes, Joseph D. Cuiffi, Stephen J. Fonash

Research output: Contribution to conference › Paper › peer-review

Abstract

The use of non-matrix laser desorption methods and MALDI-TOF-mass spectrometry (MS) for rapid drug metabolite profiling was discussed. Optimization of the refined method was carried out on microsomal incubations using nanostructured films in conjunction with a Micromass M@LDI LR instrument, in reflection mode with lock mass correction. The surface of the film was comprised of vertical, rod-like columns, bundled into larger columnar features contained in a continuous void space. The analysis of a microsomal incubation of dextromethorphan showed adequate sensitivity for detection of the parent drug and metabolite.

Original language	English (US)
Pages	493-494
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

All Science Journal Classification (ASJC) codes

Spectroscopy

Cite this

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title = "Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS",

abstract = "The use of non-matrix laser desorption methods and MALDI-TOF-mass spectrometry (MS) for rapid drug metabolite profiling was discussed. Optimization of the refined method was carried out on microsomal incubations using nanostructured films in conjunction with a Micromass M@LDI LR instrument, in reflection mode with lock mass correction. The surface of the film was comprised of vertical, rod-like columns, bundled into larger columnar features contained in a continuous void space. The analysis of a microsomal incubation of dextromethorphan showed adequate sensitivity for detection of the parent drug and metabolite.",

author = "Weston, {Daniel J.} and Grotz, {Diane E.} and Cox, {Kathleen A.} and Jose Castro-Perez and Steve Preece and Richard Tyldesley and Hilary Major and Hayes, {Daniel J.} and Cuiffi, {Joseph D.} and Fonash, {Stephen J.}",

year = "2002",

language = "English (US)",

pages = "493--494",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS

AU - Weston, Daniel J.

AU - Grotz, Diane E.

AU - Cox, Kathleen A.

AU - Castro-Perez, Jose

AU - Preece, Steve

AU - Tyldesley, Richard

AU - Major, Hilary

AU - Hayes, Daniel J.

AU - Cuiffi, Joseph D.

AU - Fonash, Stephen J.

PY - 2002

Y1 - 2002

N2 - The use of non-matrix laser desorption methods and MALDI-TOF-mass spectrometry (MS) for rapid drug metabolite profiling was discussed. Optimization of the refined method was carried out on microsomal incubations using nanostructured films in conjunction with a Micromass M@LDI LR instrument, in reflection mode with lock mass correction. The surface of the film was comprised of vertical, rod-like columns, bundled into larger columnar features contained in a continuous void space. The analysis of a microsomal incubation of dextromethorphan showed adequate sensitivity for detection of the parent drug and metabolite.

AB - The use of non-matrix laser desorption methods and MALDI-TOF-mass spectrometry (MS) for rapid drug metabolite profiling was discussed. Optimization of the refined method was carried out on microsomal incubations using nanostructured films in conjunction with a Micromass M@LDI LR instrument, in reflection mode with lock mass correction. The surface of the film was comprised of vertical, rod-like columns, bundled into larger columnar features contained in a continuous void space. The analysis of a microsomal incubation of dextromethorphan showed adequate sensitivity for detection of the parent drug and metabolite.

UR - http://www.scopus.com/inward/record.url?scp=2442701740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442701740&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442701740

SP - 493

EP - 494

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Rapid drug metabolite screening by non-matrix laser desorption and MALDI/TOF-MS

Abstract

Other

All Science Journal Classification (ASJC) codes

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