Rapid isolation of genomic DNA from animal tissues

G. Konat; G. Gantt; Iwona Hirschler-Laszkiewicz; E. L. Hogan

doi:10.1016/0014-4827(90)90200-T

Rapid isolation of genomic DNA from animal tissues

G. Konat, G. Gantt, Iwona Hirschler-Laszkiewicz, E. L. Hogan

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Abstract

A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

Original language	English (US)
Pages (from-to)	294-296
Number of pages	3
Journal	Experimental Cell Research
Volume	190
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(90)90200-T
State	Published - Oct 1990

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1016/0014-4827(90)90200-T

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title = "Rapid isolation of genomic DNA from animal tissues",

abstract = "A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.",

author = "G. Konat and G. Gantt and Iwona Hirschler-Laszkiewicz and Hogan, {E. L.}",

year = "1990",

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doi = "10.1016/0014-4827(90)90200-T",

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journal = "Experimental Cell Research",

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T1 - Rapid isolation of genomic DNA from animal tissues

AU - Konat, G.

AU - Gantt, G.

AU - Hirschler-Laszkiewicz, Iwona

AU - Hogan, E. L.

PY - 1990/10

Y1 - 1990/10

N2 - A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

AB - A simple technique for the isolation of very high molecular weight genomic DNA from animal tissues and cells is described. The method involves rapid isolation of nuclei and their embedding in agarose beads followed by extraction of lipids and proteins with SDS. The protocol does not require proteolytic digestion and the whole procedure can be completed in 1 day. The isolated DNA is digestible by restriction enzymes and free of ligase inhibitors.

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U2 - 10.1016/0014-4827(90)90200-T

DO - 10.1016/0014-4827(90)90200-T

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JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Rapid isolation of genomic DNA from animal tissues

Abstract

All Science Journal Classification (ASJC) codes

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