Rapid qualitative protease microassay (RPM)

S. Mohan; P. W.K. Ma; D. S. Luthe

doi:10.1016/j.jbbm.2005.07.002

Rapid qualitative protease microassay (RPM)

S. Mohan, P. W.K. Ma, D. S. Luthe

Plant Science

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A rapid qualitative protease microassay (RPM) was developed as an alternative to conventional assays of cysteine protease activity in HPLC fractions. Using this technique protease activity in samples could be visually determined within 5 min. The method was sensitive to 3.3 × 10 ^{- 7} U/mL of papain and detected cysteine protease activity in dilute HPLC fractions with activity of 5.4 × 10^{- 5} U/mL. Because the method monitors the decolorization of Coomassie Brilliant Blue stained substrate, it can be modified to detect other classes of proteases.

Original language	English (US)
Pages (from-to)	182-188
Number of pages	7
Journal	Journal of Biochemical and Biophysical Methods
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/j.jbbm.2005.07.002
State	Published - Sep 30 2005

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry

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10.1016/j.jbbm.2005.07.002

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title = "Rapid qualitative protease microassay (RPM)",

abstract = "A rapid qualitative protease microassay (RPM) was developed as an alternative to conventional assays of cysteine protease activity in HPLC fractions. Using this technique protease activity in samples could be visually determined within 5 min. The method was sensitive to 3.3 × 10 - 7 U/mL of papain and detected cysteine protease activity in dilute HPLC fractions with activity of 5.4 × 10- 5 U/mL. Because the method monitors the decolorization of Coomassie Brilliant Blue stained substrate, it can be modified to detect other classes of proteases.",

author = "S. Mohan and Ma, {P. W.K.} and Luthe, {D. S.}",

note = "Funding Information: This project is supported by NSF (IBN-0131328). ",

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AU - Mohan, S.

AU - Ma, P. W.K.

AU - Luthe, D. S.

N1 - Funding Information: This project is supported by NSF (IBN-0131328).

PY - 2005/9/30

Y1 - 2005/9/30

N2 - A rapid qualitative protease microassay (RPM) was developed as an alternative to conventional assays of cysteine protease activity in HPLC fractions. Using this technique protease activity in samples could be visually determined within 5 min. The method was sensitive to 3.3 × 10 - 7 U/mL of papain and detected cysteine protease activity in dilute HPLC fractions with activity of 5.4 × 10- 5 U/mL. Because the method monitors the decolorization of Coomassie Brilliant Blue stained substrate, it can be modified to detect other classes of proteases.

AB - A rapid qualitative protease microassay (RPM) was developed as an alternative to conventional assays of cysteine protease activity in HPLC fractions. Using this technique protease activity in samples could be visually determined within 5 min. The method was sensitive to 3.3 × 10 - 7 U/mL of papain and detected cysteine protease activity in dilute HPLC fractions with activity of 5.4 × 10- 5 U/mL. Because the method monitors the decolorization of Coomassie Brilliant Blue stained substrate, it can be modified to detect other classes of proteases.

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JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 3

ER -

Rapid qualitative protease microassay (RPM)

Abstract

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