TY - JOUR
T1 - Rapid screening of molecular arrays using imaging TOF-SIMS
AU - Xu, J. Y.
AU - Braun, R. M.
AU - Winograd, N.
N1 - Funding Information:
The authors would thank NIH and NSF for funding support. Thanks also goes to Abigale Marcus for her helpful discussion.
PY - 2003/1/15
Y1 - 2003/1/15
N2 - We explored the application of imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) to the high-throughput analysis needed for combinatorial chemistry research. Prototypical examples include the characterization of polymer resins, which are chemically modified as part of a combinatorial library synthesis. We studied sample conditioning for various polymer matrices, linker systems, and analytes attached to the linkers and found that the hydrophilicity of the supporting substrates play a very important role in confining the signals to a localized area. We also developed protocols for the high-throughput purpose that use specially designed substrates to hold a large number of resins (as many as 10,000), which avoids cross-contamination among components. Using this approach, we are able to perform chemical assays on polymer resins at a rate of about 1-10 s -1 . This analysis has equivalent chemical specificity and sensitivity to but a speed at least an order of magnitude faster than that of ESI-MS or LC-MS.
AB - We explored the application of imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) to the high-throughput analysis needed for combinatorial chemistry research. Prototypical examples include the characterization of polymer resins, which are chemically modified as part of a combinatorial library synthesis. We studied sample conditioning for various polymer matrices, linker systems, and analytes attached to the linkers and found that the hydrophilicity of the supporting substrates play a very important role in confining the signals to a localized area. We also developed protocols for the high-throughput purpose that use specially designed substrates to hold a large number of resins (as many as 10,000), which avoids cross-contamination among components. Using this approach, we are able to perform chemical assays on polymer resins at a rate of about 1-10 s -1 . This analysis has equivalent chemical specificity and sensitivity to but a speed at least an order of magnitude faster than that of ESI-MS or LC-MS.
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U2 - 10.1016/S0169-4332(02)00625-6
DO - 10.1016/S0169-4332(02)00625-6
M3 - Article
AN - SCOPUS:12244310128
SN - 0169-4332
VL - 203-204
SP - 201
EP - 204
JO - Applied Surface Science
JF - Applied Surface Science
ER -