TY - JOUR
T1 - Rapid serial analysis of multiple oligonucleotide samples on a microchip using optically-gated injection
AU - Roddy, Elizabeth Smith
AU - Lapos, Julie A.
AU - Ewing, Andrew G.
N1 - Funding Information:
Funding for this research was provided by the National Institutes of Health and the National Science Foundation. This work was performed in part at the Penn State Nanofabrication Facility, a member of the National Nanofabrication Users Network, which is supported by the National Science Foundation under Grant Number 33810-6190, The Pennsylvania State University and industrial affiliates.
PY - 2003/7/4
Y1 - 2003/7/4
N2 - Optically-gated injection of fluorescently-labeled DNA has been accomplished for the first time. Rapid, serial analysis of oligonucleotide ladders has been shown on a microchip using this injection technique. Separations of five- and six-component samples have been completed in 60 s or less with a capability to carry out serial injections of these samples every 15 s. The technique has been shown to have better than five base resolution for small oligonucleotides and excellent reproducibility in migration times (≤0.75% RSD). Currently, the limit of detection for the system is 0.23 μM. Additionally, multiple unique samples of DNA have been consecutively analyzed in a single separation lane using optical gating. Six consecutive injections of three different samples have been achieved with no sample carryover and a total analysis time of ∼10 min. These results show the potential of optical gating as an alternative injection technique for high-throughput DNA applications, such as genotyping and monitoring dynamic processes.
AB - Optically-gated injection of fluorescently-labeled DNA has been accomplished for the first time. Rapid, serial analysis of oligonucleotide ladders has been shown on a microchip using this injection technique. Separations of five- and six-component samples have been completed in 60 s or less with a capability to carry out serial injections of these samples every 15 s. The technique has been shown to have better than five base resolution for small oligonucleotides and excellent reproducibility in migration times (≤0.75% RSD). Currently, the limit of detection for the system is 0.23 μM. Additionally, multiple unique samples of DNA have been consecutively analyzed in a single separation lane using optical gating. Six consecutive injections of three different samples have been achieved with no sample carryover and a total analysis time of ∼10 min. These results show the potential of optical gating as an alternative injection technique for high-throughput DNA applications, such as genotyping and monitoring dynamic processes.
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U2 - 10.1016/S0021-9673(03)00239-5
DO - 10.1016/S0021-9673(03)00239-5
M3 - Article
C2 - 12929976
AN - SCOPUS:0037629663
SN - 0021-9673
VL - 1004
SP - 217
EP - 224
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -