TY - JOUR
T1 - Rapid turnover of intra-host genetic diversity in Zucchini yellow mosaic virus
AU - Simmons, Heather E.
AU - Holmes, Edward C.
AU - Stephenson, Andrew G.
N1 - Funding Information:
This work was supported by Biotechnology Risk Assessment Program Grant No. 2009-33120-20093 from the USDA National Institute of Food and Agriculture. ECH is supported in part by Grant R01 GM080533 from the National Institutes of Health . We are grateful to Joseph Dunham for his invaluable advice and assistance, and thank Melinda Bothe, Sarah Scanlon and James Parker for their help performing mini-preps.
PY - 2011/2
Y1 - 2011/2
N2 - Genetic diversity in RNA viruses is shaped by a variety of evolutionary processes, including the bottlenecks that may occur at inter-host transmission. However, how these processes structure genetic variation at the scale of individual hosts is only partly understood. We obtained intra-host sequence data for the coat protein (CP) gene of Zucchini yellow mosaic virus (ZYMV) from two horizontally transmitted populations - one via aphid, the other without - and with multiple samples from individual plants. We show that although mutations are generated relatively frequently within infected plants, attaining similar levels of genetic diversity to that seen in some animal RNA viruses (mean intra-sample diversity of 0.02%), most mutations are likely to be transient, deleterious, and purged rapidly. We also observed more population structure in the aphid transmitted viral population, including the same mutations in multiple clones, the presence of a sub-lineage, and evidence for the short-term complementation of defective genomes.
AB - Genetic diversity in RNA viruses is shaped by a variety of evolutionary processes, including the bottlenecks that may occur at inter-host transmission. However, how these processes structure genetic variation at the scale of individual hosts is only partly understood. We obtained intra-host sequence data for the coat protein (CP) gene of Zucchini yellow mosaic virus (ZYMV) from two horizontally transmitted populations - one via aphid, the other without - and with multiple samples from individual plants. We show that although mutations are generated relatively frequently within infected plants, attaining similar levels of genetic diversity to that seen in some animal RNA viruses (mean intra-sample diversity of 0.02%), most mutations are likely to be transient, deleterious, and purged rapidly. We also observed more population structure in the aphid transmitted viral population, including the same mutations in multiple clones, the presence of a sub-lineage, and evidence for the short-term complementation of defective genomes.
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U2 - 10.1016/j.virusres.2010.11.007
DO - 10.1016/j.virusres.2010.11.007
M3 - Article
C2 - 21138748
AN - SCOPUS:78751702723
SN - 0168-1702
VL - 155
SP - 389
EP - 396
JO - Virus Research
JF - Virus Research
IS - 2
ER -