Abstract
We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3884-3890 |
| Number of pages | 7 |
| Journal | Molecular and cellular biology |
| Volume | 6 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1986 |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
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