Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission

Lixin Zang; Huimin Zhao; Jianyu Hua; Feng Qin; Yangdong Zheng; Zhiguo Zhang; Wenwu Cao

doi:10.1016/j.snb.2016.03.072

Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission

Lixin Zang, Huimin Zhao, Jianyu Hua, Feng Qin, Yangdong Zheng, Zhiguo Zhang, Wenwu Cao

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (I_nitrogen/I_oxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.

Original language	English (US)
Pages (from-to)	539-546
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	231
DOIs	https://doi.org/10.1016/j.snb.2016.03.072
State	Published - Aug 1 2016

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2016.03.072

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title = "Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission",

abstract = "In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.",

author = "Lixin Zang and Huimin Zhao and Jianyu Hua and Feng Qin and Yangdong Zheng and Zhiguo Zhang and Wenwu Cao",

note = "Funding Information: This research was supported in part by the National Key Basic Research Program of China (973 Program, Grant No. 2013CB632900 ), the National Natural Science Foundation of China (Grant No. 61308065 ) and the National Natural Science Foundation of China (Grant No. 81530052 ). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V. All rights reserved.",

year = "2016",

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doi = "10.1016/j.snb.2016.03.072",

language = "English (US)",

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T1 - Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission

AU - Zang, Lixin

AU - Zhao, Huimin

AU - Hua, Jianyu

AU - Qin, Feng

AU - Zheng, Yangdong

AU - Zhang, Zhiguo

AU - Cao, Wenwu

N1 - Funding Information: This research was supported in part by the National Key Basic Research Program of China (973 Program, Grant No. 2013CB632900 ), the National Natural Science Foundation of China (Grant No. 61308065 ) and the National Natural Science Foundation of China (Grant No. 81530052 ). Publisher Copyright: © 2016 Elsevier B.V. All rights reserved.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.

AB - In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.

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Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission

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