TY - JOUR
T1 - Ratiometric dissolved oxygen sensitive indicator based on lutetium labeled hematoporphyrin monomethyl ether with balanced phosphorescence and fluorescence dual emission
AU - Zang, Lixin
AU - Zhao, Huimin
AU - Hua, Jianyu
AU - Qin, Feng
AU - Zheng, Yangdong
AU - Zhang, Zhiguo
AU - Cao, Wenwu
N1 - Funding Information:
This research was supported in part by the National Key Basic Research Program of China (973 Program, Grant No. 2013CB632900 ), the National Natural Science Foundation of China (Grant No. 61308065 ) and the National Natural Science Foundation of China (Grant No. 81530052 ).
Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.
AB - In order to improve the signal to noise ratio in ratiometric oxygen sensing, comparable phosphorescence and fluorescence intensities are required. To balance the room temperature phosphorescence (RTP) and fluorescence intensities, lutetium labeled hematoporphyrin monomethyl ether (Lu-HMME) was used and its luminescence and oxygen quenching properties were studied. Luminescence spectrum indicates that RTP and fluorescence intensities of Lu-HMME are in the same order of magnitude in air-saturated solution. As expected, the fluorescence emission of Lu-HMME was independent of dissolved oxygen while RTP emission was strongly dependent on oxygen. A compact and simple ratiometric oxygen measurement system based on Lu-HMME was constructed utilizing fiber optoelectronic sensing device. The intensity response (Initrogen/Ioxygen) of Lu-HMME RTP to dissolved oxygen is higher than 4.6. The measurement uncertainty of 0.06 μM in air-saturated methanol solution was achieved using Lu-HMME. Our results suggest a high accuracy method for ratiometric dissolved oxygen measurement based on balanced phosphorescence and fluorescence emissions.
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U2 - 10.1016/j.snb.2016.03.072
DO - 10.1016/j.snb.2016.03.072
M3 - Article
AN - SCOPUS:84961574374
SN - 0925-4005
VL - 231
SP - 539
EP - 546
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -