Reactive intermediates in cytochrome P450 catalysis

Courtney M. Krest, Elizabeth L. Onderko, Timothy H. Yosca, Julio C. Calixto, Richard F. Karp, Jovan Livada, Jonathan Rittle, Michael T. Green

Research output: Contribution to journalShort surveypeer-review

163 Scopus citations


Recently, we reported the spectroscopic and kinetic characterizations of cytochrome P450 compound I in CYP119A1, effectively closing the catalytic cycle of cytochrome P450-mediated hydroxylations. In this minireview, we focus on the developments that made this breakthrough possible. We examine the importance of enzyme purification in the quest for reactive intermediates and report the preparation of compound I in a second P450 (P450ST). In an effort to bring clarity to the field, we also examine the validity of controversial reports claiming the production of P450 compound I through the use of peroxynitrite and laser flash photolysis.

Original languageEnglish (US)
Pages (from-to)17074-17081
Number of pages8
JournalJournal of Biological Chemistry
Issue number24
StatePublished - Jun 14 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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