TY - JOUR
T1 - Reagent-and separation-free measurements of urine creatinine concentration using stamping surface enhanced Raman scattering (S-SERS)
AU - Li, Ming
AU - Du, Yong
AU - Zhao, Fusheng
AU - Zeng, Jianbo
AU - Mohan, Chandra
AU - Shih, Wei Chuan
N1 - Publisher Copyright:
© 2015 Optical Society of America.
PY - 2015
Y1 - 2015
N2 - We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.
AB - We report a novel reagent-and separation-free method for urine creatinine concentration measurement using stamping surface enhanced Raman scattering (S-SERS) technique with nanoporous gold disk (NPGD) plasmonic substrates, a label-free, multiplexed molecular sensing and imaging technique recently developed by us. The performance of this new technology is evaluated by the detection and quantification of creatinine spiked in three different liquids: creatinine in water, mixture of creatinine and urea in water, and creatinine in artificial urine within physiologically relevant concentration ranges. Moreover, the potential application of our method is demonstrated by creatinine concentration measurements in urine samples collected from a mouse model of nephritis. The limit of detection of creatinine was 13.2 nM (0.15 μg/dl) and 0.68 mg/dl in water and urine, respectively. Our method would provide an alternative tool for rapid, costeffective, and reliable urine analysis for non-invasive diagnosis and monitoring of renal function.
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U2 - 10.1364/BOE.6.000849
DO - 10.1364/BOE.6.000849
M3 - Article
AN - SCOPUS:84942365817
SN - 2156-7085
VL - 6
SP - 849
EP - 858
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 3
ER -