Abstract
This study presents a novel approach to probe in situ, real-time changes in mixed-species biofilms via quartz crystal microbalance with dissipation (QCM-D). The viscoelasticity of a model wastewater biofilm was monitored by QCM-D, and quantitative changes in both measured frequency, ∆f, and dissipation, ∆D, reflected biofilm structural changes in the presence of silver nanoparticles (Ag-NPs). Complimentary results from confocal laser scanning microscopy confirmed biofilm attachment and the presence of live cells. A step-input experiment with increasing concentrations of Ag-NPs (127, 666, 1160, 1632 ppb) was performed. A Voigt model analysis of QCM-D results shows differing biofilm viscoelastic behavior as a function of Ag-NP concentration. The step input response of ∆D/∆f ratio showed that Ag-NPs cause a structural pattern of firmness at low concentrations (up to 666 ppb), and less rigidity of attached mass at higher concentrations (up to 1632 ppb) for the mixed species biofilm studied.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 49-53 |
| Number of pages | 5 |
| Journal | Colloids and Interface Science Communications |
| Volume | 28 |
| DOIs | |
| State | Published - Jan 2019 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Colloid and Surface Chemistry
- Materials Chemistry