TY - JOUR
T1 - Recent advances in computational protein design
AU - Pantazes, Robert J.
AU - Grisewood, Matthew J.
AU - Maranas, Costas D.
N1 - Funding Information:
This work was funded by the National Science Foundation [CBET-0639962] .
PY - 2011/8
Y1 - 2011/8
N2 - Proteins are the molecules cells primarily rely on for catalysis, recognition, signaling, defense, locomotion, and structural integrity. Engineering proteins for improved function or new applications is a fast-growing segment of biotechnology and biomedicine. Experimental efforts based on the screening of large mutant libraries have led to many successes but they do not provide quantitative design principles and/or insight into the structural features that underpin the desired function. The computational de novo design of proteins promises to bridge this gap; however, it requires reliable structure prediction, provisions for protein stability, and accurate descriptions of inter-molecule interactions. Studies that successfully meet all these criteria are beginning to emerge including the design of an O 2-binding protein and a novel enzyme that catalyzes a Diels-Alder reaction.
AB - Proteins are the molecules cells primarily rely on for catalysis, recognition, signaling, defense, locomotion, and structural integrity. Engineering proteins for improved function or new applications is a fast-growing segment of biotechnology and biomedicine. Experimental efforts based on the screening of large mutant libraries have led to many successes but they do not provide quantitative design principles and/or insight into the structural features that underpin the desired function. The computational de novo design of proteins promises to bridge this gap; however, it requires reliable structure prediction, provisions for protein stability, and accurate descriptions of inter-molecule interactions. Studies that successfully meet all these criteria are beginning to emerge including the design of an O 2-binding protein and a novel enzyme that catalyzes a Diels-Alder reaction.
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U2 - 10.1016/j.sbi.2011.04.005
DO - 10.1016/j.sbi.2011.04.005
M3 - Review article
C2 - 21600758
AN - SCOPUS:80052080451
SN - 0959-440X
VL - 21
SP - 467
EP - 472
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 4
ER -