TY - JOUR
T1 - Recent and emerging innovations in Salmonella detection
T2 - A food and environmental perspective
AU - Bell, Rebecca L.
AU - Jarvis, Karen G.
AU - Ottesen, Andrea R.
AU - Mcfarland, Melinda A.
AU - Brown, Eric W.
N1 - Publisher Copyright:
© 2016 John Wiley & Sons Ltd and Society for Applied Microbiology.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Salmonella is a diverse genus of Gram-negative bacilli and a major foodborne pathogen responsible for more than a million illnesses annually in the United States alone. Rapid, reliable detection and identification of this pathogen in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators. Unfortunately, the time to result is too long to effectively monitor foodstuffs, especially those with very short shelf lives. Advances in traditional microbiology and molecular biology over the past 25 years have greatly improved the speed at which this pathogen is detected. Nonetheless, food and environmental samples possess a distinctive set of challenges for these newer, more rapid methodologies. Furthermore, more detailed identification and subtyping strategies still rely heavily on the availability of a pure isolate. However, major shifts in DNA sequencing technologies are meeting this challenge by advancing the detection, identification and subtyping of Salmonella towards a culture-independent diagnostic framework. This review will focus on current approaches and state-of-the-art next-generation advances in the detection, identification and subtyping of Salmonella from food and environmental sources. Rapid, reliable detection and identification of Salmonella in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators; unfortunately the time to result is too long to effectively monitor foodstuffs. This review will focus on current approaches and state-of-the-art next generation advances in molecular biology for the detection, identification, and subtyping of Salmonella from food and environmental sources.
AB - Salmonella is a diverse genus of Gram-negative bacilli and a major foodborne pathogen responsible for more than a million illnesses annually in the United States alone. Rapid, reliable detection and identification of this pathogen in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators. Unfortunately, the time to result is too long to effectively monitor foodstuffs, especially those with very short shelf lives. Advances in traditional microbiology and molecular biology over the past 25 years have greatly improved the speed at which this pathogen is detected. Nonetheless, food and environmental samples possess a distinctive set of challenges for these newer, more rapid methodologies. Furthermore, more detailed identification and subtyping strategies still rely heavily on the availability of a pure isolate. However, major shifts in DNA sequencing technologies are meeting this challenge by advancing the detection, identification and subtyping of Salmonella towards a culture-independent diagnostic framework. This review will focus on current approaches and state-of-the-art next-generation advances in the detection, identification and subtyping of Salmonella from food and environmental sources. Rapid, reliable detection and identification of Salmonella in food and environmental sources is key to safeguarding the food supply. Traditional microbiological culture techniques have been the 'gold standard' for State and Federal regulators; unfortunately the time to result is too long to effectively monitor foodstuffs. This review will focus on current approaches and state-of-the-art next generation advances in molecular biology for the detection, identification, and subtyping of Salmonella from food and environmental sources.
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U2 - 10.1111/1751-7915.12359
DO - 10.1111/1751-7915.12359
M3 - Short survey
C2 - 27041363
AN - SCOPUS:84963593813
SN - 1751-7907
VL - 9
SP - 279
EP - 292
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 3
ER -