Recombinant JC viral DNA: Verification and physical map of prototype

Jonathan D. Martin; Karl H. Brackmann; Brian W. Grinnell; Richard J. Frisque; Duard L. Walker; Maurice Green

doi:10.1016/0006-291X(82)91567-4

Recombinant JC viral DNA: Verification and physical map of prototype

Jonathan D. Martin, Karl H. Brackmann, Brian W. Grinnell, Richard J. Frisque, Duard L. Walker, Maurice Green

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Genomic DNA of the human polyomavirus JC was molecularly cloned from DNA extracted from primary human fetal glial cells infected with prototype (MAD-1) virus and from diseased brain tissue from which JC virus originally was isolated. This report documents that these recombinant MAD-1 DNAs are identical, are prototypical, and may serve as unambiguous references to distinguish the variant DNAs produced by independent isolates of JC virus. Possible ambiguity in other recombinants of MAD-1 DNA is discussed. A new restriction endonuclease cleavage map of MAD-1 DNA was derived for 52 sites cleaved by eight enzymes.

Original language	English (US)
Pages (from-to)	70-77
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	109
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(82)91567-4
State	Published - Nov 16 1982

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(82)91567-4

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abstract = "Genomic DNA of the human polyomavirus JC was molecularly cloned from DNA extracted from primary human fetal glial cells infected with prototype (MAD-1) virus and from diseased brain tissue from which JC virus originally was isolated. This report documents that these recombinant MAD-1 DNAs are identical, are prototypical, and may serve as unambiguous references to distinguish the variant DNAs produced by independent isolates of JC virus. Possible ambiguity in other recombinants of MAD-1 DNA is discussed. A new restriction endonuclease cleavage map of MAD-1 DNA was derived for 52 sites cleaved by eight enzymes.",

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AU - Martin, Jonathan D.

AU - Brackmann, Karl H.

AU - Grinnell, Brian W.

AU - Frisque, Richard J.

AU - Walker, Duard L.

AU - Green, Maurice

PY - 1982/11/16

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AB - Genomic DNA of the human polyomavirus JC was molecularly cloned from DNA extracted from primary human fetal glial cells infected with prototype (MAD-1) virus and from diseased brain tissue from which JC virus originally was isolated. This report documents that these recombinant MAD-1 DNAs are identical, are prototypical, and may serve as unambiguous references to distinguish the variant DNAs produced by independent isolates of JC virus. Possible ambiguity in other recombinants of MAD-1 DNA is discussed. A new restriction endonuclease cleavage map of MAD-1 DNA was derived for 52 sites cleaved by eight enzymes.

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Recombinant JC viral DNA: Verification and physical map of prototype

Abstract

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