TY - JOUR
T1 - Recombinogenic targeting
T2 - A new approach to genomic analysis - A review
AU - Shashikant, Cooduvalli S.
AU - L. Carr, Janet
AU - Bhargava, Jaya
AU - Bentley, Kevin L.
AU - Ruddle, Frank H.
N1 - Funding Information:
This work is supported by NIH grant GM09966 to F.H.R.
PY - 1998/11/26
Y1 - 1998/11/26
N2 - Currently, recombinational cloning procedures based upon methods developed for yeast, Saccharomyces cerevisiae, are being exploited for targeted cloning and in-vivo modification of genomic clones. In this review, we will discuss the development of large-insert vectors, homologous recombination-based techniques for cloning and modification, and their application towards functional analysis of genes using transgenic mouse model systems.
AB - Currently, recombinational cloning procedures based upon methods developed for yeast, Saccharomyces cerevisiae, are being exploited for targeted cloning and in-vivo modification of genomic clones. In this review, we will discuss the development of large-insert vectors, homologous recombination-based techniques for cloning and modification, and their application towards functional analysis of genes using transgenic mouse model systems.
UR - http://www.scopus.com/inward/record.url?scp=0032569968&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032569968&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(98)00369-2
DO - 10.1016/S0378-1119(98)00369-2
M3 - Article
C2 - 9858667
AN - SCOPUS:0032569968
SN - 0378-1119
VL - 223
SP - 9
EP - 20
JO - Gene
JF - Gene
IS - 1-2
ER -