TY - JOUR
T1 - Recruitment of coregulator complexes to the β-globin gene locus by TFII-I and upstream stimulatory factor
AU - Crusselle-Davis, Valerie J.
AU - Zhou, Zhuo
AU - Anantharaman, Archana
AU - Moghimi, Babak
AU - Dodev, Tihomir
AU - Huang, Suming
AU - Bungert, Jörg
PY - 2007/12
Y1 - 2007/12
N2 - Upstream stimulatory factor and TFII-I are ubiquitously expressed helix-loop-helix transcription factors that interact with E-box sequences and or initiator elements. We previously demonstrated that upstream stimulatory factor is an activator of β-globin gene expression whereas TFII-I is a repressor. In the present study, we demonstrate that upstream stimulatory factor interacts with the coactivator p300 and that this interaction is restricted to erythroid cells expressing the adult β-globin gene. Furthermore, we demonstrate that Suz12, a component of the polycomb repressor complex 2, is recruited to the β-globin gene. Reducing expression of Suz12 significantly activates β-globin gene expression in an erythroid cell line with an embryonic phenotype. Suz12 also interacts with the adult β-globin gene during early stages of erythroid differentiation of mouse embryonic stem cells. Our data suggest that TFII-I contributes to the recruitment of the polycomb repressor complex 2 complex to the β-globin gene. Together, these data demonstrate that the antagonistic activities of upstream stimulatory factor and TFII-I on β-globin gene expression are mediated at least in part by protein complexes that render the promoter associated chromatin accessible or inaccessible for the transcription complex.
AB - Upstream stimulatory factor and TFII-I are ubiquitously expressed helix-loop-helix transcription factors that interact with E-box sequences and or initiator elements. We previously demonstrated that upstream stimulatory factor is an activator of β-globin gene expression whereas TFII-I is a repressor. In the present study, we demonstrate that upstream stimulatory factor interacts with the coactivator p300 and that this interaction is restricted to erythroid cells expressing the adult β-globin gene. Furthermore, we demonstrate that Suz12, a component of the polycomb repressor complex 2, is recruited to the β-globin gene. Reducing expression of Suz12 significantly activates β-globin gene expression in an erythroid cell line with an embryonic phenotype. Suz12 also interacts with the adult β-globin gene during early stages of erythroid differentiation of mouse embryonic stem cells. Our data suggest that TFII-I contributes to the recruitment of the polycomb repressor complex 2 complex to the β-globin gene. Together, these data demonstrate that the antagonistic activities of upstream stimulatory factor and TFII-I on β-globin gene expression are mediated at least in part by protein complexes that render the promoter associated chromatin accessible or inaccessible for the transcription complex.
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U2 - 10.1111/j.1742-4658.2007.06128.x
DO - 10.1111/j.1742-4658.2007.06128.x
M3 - Article
C2 - 17970752
AN - SCOPUS:36248987150
SN - 1742-464X
VL - 274
SP - 6065
EP - 6073
JO - FEBS Journal
JF - FEBS Journal
IS - 23
ER -