Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

Michael W. Epperly; Xichen Zhang; Renee Fisher; Wen Hou; Darcy Franicola; Donna Shields; Michael Quickel; Pamela Hankey-Giblin; Hong Wang; Joel S. Greenberger

doi:10.21873/invivo.11061

Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

Michael W. Epperly, Xichen Zhang, Renee Fisher, Wen Hou, Darcy Franicola, Donna Shields, Michael Quickel, Pamela Hankey-Giblin, Hong Wang, Joel S. Greenberger

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2^r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2^r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2^r C57BL/6 mice. Furthermore, Fv-2^r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2^r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

Original language	English (US)
Pages (from-to)	313-320
Number of pages	8
Journal	In Vivo
Volume	31
Issue number	3
DOIs	https://doi.org/10.21873/invivo.11061
State	Published - May 1 2017

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
Cancer Research
Pharmacology

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@article{92c0c51fab204dc293c2460d52f4ad82,

title = "Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice",

abstract = "Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.",

author = "Epperly, {Michael W.} and Xichen Zhang and Renee Fisher and Wen Hou and Darcy Franicola and Donna Shields and Michael Quickel and Pamela Hankey-Giblin and Hong Wang and Greenberger, {Joel S.}",

note = "Funding Information: The present study was supported by Grant from the NIH/NIAID U19-A168021 and the Fanconi Anemia Research Fund. This project used the UPCI animal facility that is supported in part by award P30CA047904.",

year = "2017",

month = may,

day = "1",

doi = "10.21873/invivo.11061",

language = "English (US)",

volume = "31",

pages = "313--320",

journal = "In Vivo",

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TY - JOUR

T1 - Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

AU - Epperly, Michael W.

AU - Zhang, Xichen

AU - Fisher, Renee

AU - Hou, Wen

AU - Franicola, Darcy

AU - Shields, Donna

AU - Quickel, Michael

AU - Hankey-Giblin, Pamela

AU - Wang, Hong

AU - Greenberger, Joel S.

N1 - Funding Information: The present study was supported by Grant from the NIH/NIAID U19-A168021 and the Fanconi Anemia Research Fund. This project used the UPCI animal facility that is supported in part by award P30CA047904.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

AB - Background/Aim: The polycythemia form of Friend leukemia virus (FVP) causes splenomegaly and lethal erythroleukemia in Fv-2s-susceptible mouse strains. We sought to determine whether the hematopoietic stem cell (HSC) pool was expanded in Fv-2r-resistant mice. Materials and Methods: The 120-day bone marrow transplantation competitive repopulation assay was used to determine whether FVP-infected Fv-2r C57BL/6 mice demonstrated expansion of the HSC pool compared to the pool of committed hematopoietic progenitor cells in the same marrow assayed in vitro. Results: There was a significant expansion of committed hematopoietic progenitors observed in virus-infected Fv-2s FVB mice, but not Fv-2r C57BL/6 mice. Furthermore, Fv-2r mice showed no detectable expansion of either committed hematopoietic progenitor cells or the multipotential stem cell pool by competitive repopulation assay. Conclusion: Friend virus disease in Fv-2s mice is associated with expansion of committed hematopoietic progenitors. Fv-2r mice show no expansion of either committed progenitor or pluripotential stem cell numbers.

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Reduced competitive repopulation capacity of multipotential hematopoietic stem cells in the bone marrow of friend virus-infected Fv2-resistant mice

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