TY - JOUR
T1 - Reevaluation of the evolutionary events within recA/RAD51 phylogeny
AU - Chintapalli, Sree V.
AU - Bhardwaj, Gaurav
AU - Babu, Jagadish
AU - Hadjiyianni, Loukia
AU - Hong, Yoojin
AU - Todd, George K.
AU - Boosalis, Casey A.
AU - Zhang, Zhenhai
AU - Zhou, Xiaofan
AU - Ma, Hong
AU - Anishkin, Andriy
AU - van Rossum, Damian B.
AU - Patterson, Randen L.
N1 - Funding Information:
This work was supported by the Searle Young Investigators Award and start-up money from UC Davis (RLP), and The National Institutes of Health R01 GM087410-01 (RLP). This project was also funded by a Fellowship from the Eberly College of Sciences and the Huck Institutes of the Life Sciences (DVR) and a grant with the Pennsylvania Department of Health using Tobacco Settlement Funds (DVR). The Department of Health specifically disclaims responsibility for any analyses, interpretations or conclusions. We would especially like to thank Eddie Holmes for his generous help and his valuable comments throughout this work. We also thank Maia Rabaa, Ngai Lam Ho, Rocky Boosalis, Natasha Shah, and Alyssa Thunen for their help and support during the project, as well as Jason Holmes at The Pennsylvania State University CAC center for technical assistance. We would like to thank Dr. Robert E. Rothe, Barbara Van Rossum, and Jim White, for creative dialogue.
PY - 2013/4/10
Y1 - 2013/4/10
N2 - Background: The recA/RAD51 gene family encodes a diverse set of recombinase proteins that affect homologous recombination, DNA-repair, and genome stability. The recA gene family is expressed across all three domains of life - Eubacteria, Archaea, and Eukaryotes - and even in some viruses. To date, efforts to resolve the deep evolutionary origins of this ancient protein family have been hindered by the high sequence divergence between paralogous groups (i.e. ~30% average pairwise identity).Results: Through large taxon sampling and the use of a phylogenetic algorithm designed for inferring evolutionary events in highly divergent paralogs, we obtained a robust, parsimonious and more refined phylogenetic history of the recA/RAD51 superfamily.Conclusions: In summary, our model for the evolution of recA/RAD51 family provides a better understanding of the ancient origin of recA proteins and the multiple events that lead to the diversification of recA homologs in eukaryotes, including the discovery of additional RAD51 sub-families.
AB - Background: The recA/RAD51 gene family encodes a diverse set of recombinase proteins that affect homologous recombination, DNA-repair, and genome stability. The recA gene family is expressed across all three domains of life - Eubacteria, Archaea, and Eukaryotes - and even in some viruses. To date, efforts to resolve the deep evolutionary origins of this ancient protein family have been hindered by the high sequence divergence between paralogous groups (i.e. ~30% average pairwise identity).Results: Through large taxon sampling and the use of a phylogenetic algorithm designed for inferring evolutionary events in highly divergent paralogs, we obtained a robust, parsimonious and more refined phylogenetic history of the recA/RAD51 superfamily.Conclusions: In summary, our model for the evolution of recA/RAD51 family provides a better understanding of the ancient origin of recA proteins and the multiple events that lead to the diversification of recA homologs in eukaryotes, including the discovery of additional RAD51 sub-families.
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U2 - 10.1186/1471-2164-14-240
DO - 10.1186/1471-2164-14-240
M3 - Article
C2 - 23574621
AN - SCOPUS:84875946720
SN - 1471-2164
VL - 14
JO - BMC genomics
JF - BMC genomics
IS - 1
M1 - 240
ER -