TY - JOUR
T1 - Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum
AU - Yuan, Lili
AU - Hao, Mingming
AU - Wu, Lanou
AU - Zhao, Zhen
AU - Rosenthal, Benjamin M.
AU - Li, Xiaomei
AU - He, Yongshu
AU - Sun, Ling
AU - Feng, Guohua
AU - Xiang, Zheng
AU - Cui, Liwang
AU - Yang, Zhaoqing
N1 - Funding Information:
We thank Dr. Jun Miao for technical advice. This project was funded by the National Institute of Health ( U19 AI089672 to L.C., 1R01AI075429 to Z.Y.), National Science Foundation of China (Nos. U1202226 , 81161120421 and No. 31260508 to Z.Y.), the Doctor Science Research Foundation of the Education Ministry of China (No. 20125317110001 to Z.Y.) and Yunnan Science Foundation (No. 2012FB153 to Z.X.).
PY - 2014/5
Y1 - 2014/5
N2 - Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.
AB - Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4°C for 8-24h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were performed using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled.
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U2 - 10.1016/j.exppara.2014.03.010
DO - 10.1016/j.exppara.2014.03.010
M3 - Article
C2 - 24632190
AN - SCOPUS:84896972291
SN - 0014-4894
VL - 140
SP - 18
EP - 23
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1
ER -