A derivative of Sepharose4B, p-(N-acetyl-l-tyrosineazo)benzamidoethyl Sepharose4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-l-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Organic Chemistry