Regenerable affinity chromatography support

V. K. Joshi; K. M. Shahani; A. Kilara; Fred W. Wagner

doi:10.1016/S0021-9673(00)92081-8

Regenerable affinity chromatography support

V. K. Joshi, K. M. Shahani, A. Kilara, Fred W. Wagner

Food Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A derivative of Sepharose4B, p-(N-acetyl-l-tyrosineazo)benzamidoethyl Sepharose4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-l-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.

Original language	English (US)
Pages (from-to)	11-18
Number of pages	8
Journal	Journal of Chromatography A
Volume	176
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(00)92081-8
State	Published - Aug 1 1979

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)92081-8

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author = "Joshi, {V. K.} and Shahani, {K. M.} and A. Kilara and Wagner, {Fred W.}",

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T1 - Regenerable affinity chromatography support

AU - Joshi, V. K.

AU - Shahani, K. M.

AU - Kilara, A.

AU - Wagner, Fred W.

PY - 1979/8/1

Y1 - 1979/8/1

N2 - A derivative of Sepharose4B, p-(N-acetyl-l-tyrosineazo)benzamidoethyl Sepharose4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-l-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.

AB - A derivative of Sepharose4B, p-(N-acetyl-l-tyrosineazo)benzamidoethyl Sepharose4B, was synthesized and used for the selective immobilization of proteins. The azo bond of this derivative can be reduced to liberate p-aminobenzamidoethyl Sepharose 4B, which can be diazotized and recoupled to N-acetyl-l-tyrosine and subsequently to a fresh preparation of protein. This regenerable affinity chromatography support was regenerated successively through five cycles without detectable loss of functional groups.

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SP - 11

EP - 18

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1

ER -

Regenerable affinity chromatography support

Abstract

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