TY - JOUR
T1 - Regulation of interleukin-8 expression in melanoma-stimulated neutrophil inflammatory response
AU - Peng, Hsin Hsin
AU - Liang, Shile
AU - Henderson, Andrew J.
AU - Dong, Cheng
N1 - Funding Information:
Authors thank Dr. Avery August (Penn State University, Department of Veterinary and Biomedical Sciences) for helpful discussions. The Authors are grateful for the support of the Penn State General Clinical Research Center (GCRC) staff who provided nursing care. This work was supported by NSF-BES0138474, NIH-CA 097306, NIH-AI 062467, Grace Woodward Collaborative Research Fund and Johnson & Johnson Innovative Technology Research Fund, and the PA Department of Health Research Fund (SAP #41000-26343).
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Inflammation facilitates tumor progression including metastasis. Interleukin-8 (IL-8) is a chemokine that regulates polymorphonuclear neutrophil (PMN) mobilization and activity and we hypothesize that this cytokine influences tumor behavior. We have demonstrated that IL-8 is crucial for PMN-mediated melanoma extravasation under flow conditions. In addition, IL-8 is up-regulated in PMNs upon co-culturing with melanoma cells. Melanoma cells induce IκB-α degradation in PMNs indicating that NF-κB signaling is active in PMNs. Furthermore, the production of IL-8 in PMNs is NF-κB dependent. We have further identified that interleukin-6 (IL-6) and interleukin-1β (IL-1β) from PMN-melanoma co-cultures synergistically contribute to IκB-α degradation and IL-8 synthesis in PMNs. Taken together, these findings show that melanoma cells induce PMNs to secrete IL-8 through activation of NF-κB and suggest a model in which this interaction promotes a microenvironment that is favorable for metastasis.
AB - Inflammation facilitates tumor progression including metastasis. Interleukin-8 (IL-8) is a chemokine that regulates polymorphonuclear neutrophil (PMN) mobilization and activity and we hypothesize that this cytokine influences tumor behavior. We have demonstrated that IL-8 is crucial for PMN-mediated melanoma extravasation under flow conditions. In addition, IL-8 is up-regulated in PMNs upon co-culturing with melanoma cells. Melanoma cells induce IκB-α degradation in PMNs indicating that NF-κB signaling is active in PMNs. Furthermore, the production of IL-8 in PMNs is NF-κB dependent. We have further identified that interleukin-6 (IL-6) and interleukin-1β (IL-1β) from PMN-melanoma co-cultures synergistically contribute to IκB-α degradation and IL-8 synthesis in PMNs. Taken together, these findings show that melanoma cells induce PMNs to secrete IL-8 through activation of NF-κB and suggest a model in which this interaction promotes a microenvironment that is favorable for metastasis.
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U2 - 10.1016/j.yexcr.2006.10.030
DO - 10.1016/j.yexcr.2006.10.030
M3 - Article
C2 - 17141217
AN - SCOPUS:33846580105
SN - 0014-4827
VL - 313
SP - 551
EP - 559
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 3
ER -