TY - JOUR
T1 - Regulation of prostaglandin synthesis by progesterone in the bovine corpus luteum
AU - Pate, Joy L.
N1 - Funding Information:
The author wishes to express her gratitude to Ms. Leslie S. Jones for her excellent technical assistance, and to Michelle Vernier for her aid in the preparation of this manuscript. The donations of prostaglandin (Dr. R. Fertel) and progesterone (Dr. G. Niswender) antisera are greatly appreciated. This study was supported by a grant from the United States Department of Agriculture, Competitive Research Grants Program. Salaries and research support provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, Journal Article No. 64-88.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988/9
Y1 - 1988/9
N2 - The objective of the present study was to investigate the influence of progesterone on prostaglandin synthesis by the corpus luteum (CL). Corpora lutea were obtained from dairy cows on days 4,6,10, and 18 of the estrous cycle, dissociated. and placed in serum-free culture. The addition of luteinizing hormone (LH) resulted in a slight, but nonsignificant (p > 0.05), increase in levels of 6-keto-PGF1α, and had no effect on PGF2α. Progesterone treatment caused a significant, dose-dependent decrease in both PGF2α and 6-keto-PGF1α in 6-day and 10-day corpora lutea, but not in 4-day or 18-day corpora lutea. In the 6- and 10-day corpora lutea, progesterone treatment resulted in a greater inhibition of PGF2α than 6-keto-PGF1α production. Therefore, progesterone treatment brought about an increase in the 6-keto-PGF1α to PGF2α ratio in these cells (12.9 vs. 21.3). It is concluded from these studies that progesterone can modulate luteal prostacyclin and PGF2α synthesis, suggesting an interaction of progesterone and prostaglandin production within the corpus luteum.
AB - The objective of the present study was to investigate the influence of progesterone on prostaglandin synthesis by the corpus luteum (CL). Corpora lutea were obtained from dairy cows on days 4,6,10, and 18 of the estrous cycle, dissociated. and placed in serum-free culture. The addition of luteinizing hormone (LH) resulted in a slight, but nonsignificant (p > 0.05), increase in levels of 6-keto-PGF1α, and had no effect on PGF2α. Progesterone treatment caused a significant, dose-dependent decrease in both PGF2α and 6-keto-PGF1α in 6-day and 10-day corpora lutea, but not in 4-day or 18-day corpora lutea. In the 6- and 10-day corpora lutea, progesterone treatment resulted in a greater inhibition of PGF2α than 6-keto-PGF1α production. Therefore, progesterone treatment brought about an increase in the 6-keto-PGF1α to PGF2α ratio in these cells (12.9 vs. 21.3). It is concluded from these studies that progesterone can modulate luteal prostacyclin and PGF2α synthesis, suggesting an interaction of progesterone and prostaglandin production within the corpus luteum.
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U2 - 10.1016/0090-6980(88)90072-X
DO - 10.1016/0090-6980(88)90072-X
M3 - Article
C2 - 3237995
AN - SCOPUS:0023722839
SN - 0090-6980
VL - 36
SP - 303
EP - 315
JO - Prostaglandins
JF - Prostaglandins
IS - 3
ER -