@article{db9ae709b0d24f2f9bc61ac58461b02e,
title = "Regulation of T cell development by the deubiquitinating enzyme CYLD",
abstract = "T cell receptor signaling is essential for the generation and maturation of T lymphocyte precursors. Here we identify the deubiquitinating enzyme CYLD as a positive regulator of proximal T cell receptor signaling in thymocytes. CYLD physically interacted with active Lck and promoted recruitment of active Lck to its substrate, Zap70. CYLD also removed both Lys 48- and Lys 63-linked polyubiquitin chains from Lck. Because of a cell-autonomous defect in T cell development, CYLD-deficient mice had substantially fewer mature CD4+ and CD8+ single-positive thymocytes and peripheral T cells.",
author = "William Reiley and Minying Zhang and Wei Jin and Mandy Losiewicz and Donohue, {Keri B.} and Norbury, {Christopher C.} and Sun, {Shao Cong}",
note = "Funding Information: We thank T. Saunders and E. Hughes of the University of Michigan Transgenic Core (Ann Arbor, Michigan) for the production of CYLD chimera mice; N. Sheaffer, A. Stanley and J. Bednarczyk of the Pennsylvania State College of Medicine Core facilities (Hershey, Pennsylvania) for assistance with flow cytometry, oligonucleotide synthesis and DNA sequencing; A. Nagy, R. Nagy and W. Abramow-Newerly of Mount Sinai Hospital (New York, New York) for the R1 embryonic stem cells; H. Band (Northwestern University, Chicago, Illinois), S. Lipkowitz (National Cancer Institute, Bethesda, Maryland) and Z. Chen (University of Texas Southwestern Medical Center, Dallas, Texas) for providing Lck, c-Cbl and ubiquitin expression vectors, respectively; and J. Lee for critical reading of the manuscript. Supported by National Institutes of Health (AI057555 to S.C.S. and M.Y.Z.; CA94922 to S.C.S.; AI056094 to C.C.N.; and C06 RR-15428-01 to the Pennsylvania State College of Medicine Animal Facility).",
year = "2006",
month = apr,
doi = "10.1038/ni1315",
language = "English (US)",
volume = "7",
pages = "411--417",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Research",
number = "4",
}