Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase by insulin

Clare E. Sample; Gene C. Ness

doi:10.1016/0006-291X(86)91196-4

Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase by insulin

Clare E. Sample, Gene C. Ness

Research output: Contribution to journal › Article › peer-review

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Abstract

In hypophysectomized - diabetic rats full restoration of hepatic HMG-CoA reductase activity required administration of both triiodothyronine and insulin. Giving triiodothyronine alone resulted in full restoration of reductase mRNA and immunoreactive protein but not of enzyme activity. Increasing the ratio of dithiothreitol to microsomal protein resulted in full HMG-CoA reductase activity. With 5mM glutathione, HMG-CoA reductase activity was 5 to 10 fold higher in non-diabetic animals. The data suggest that insulin acts by increasing the portion of HMG-CoA reductase present in its active free sulfhydryl form.

Original language	English (US)
Pages (from-to)	201-207
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(86)91196-4
State	Published - May 29 1986

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Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(86)91196-4

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abstract = "In hypophysectomized - diabetic rats full restoration of hepatic HMG-CoA reductase activity required administration of both triiodothyronine and insulin. Giving triiodothyronine alone resulted in full restoration of reductase mRNA and immunoreactive protein but not of enzyme activity. Increasing the ratio of dithiothreitol to microsomal protein resulted in full HMG-CoA reductase activity. With 5mM glutathione, HMG-CoA reductase activity was 5 to 10 fold higher in non-diabetic animals. The data suggest that insulin acts by increasing the portion of HMG-CoA reductase present in its active free sulfhydryl form.",

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AU - Ness, Gene C.

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N2 - In hypophysectomized - diabetic rats full restoration of hepatic HMG-CoA reductase activity required administration of both triiodothyronine and insulin. Giving triiodothyronine alone resulted in full restoration of reductase mRNA and immunoreactive protein but not of enzyme activity. Increasing the ratio of dithiothreitol to microsomal protein resulted in full HMG-CoA reductase activity. With 5mM glutathione, HMG-CoA reductase activity was 5 to 10 fold higher in non-diabetic animals. The data suggest that insulin acts by increasing the portion of HMG-CoA reductase present in its active free sulfhydryl form.

AB - In hypophysectomized - diabetic rats full restoration of hepatic HMG-CoA reductase activity required administration of both triiodothyronine and insulin. Giving triiodothyronine alone resulted in full restoration of reductase mRNA and immunoreactive protein but not of enzyme activity. Increasing the ratio of dithiothreitol to microsomal protein resulted in full HMG-CoA reductase activity. With 5mM glutathione, HMG-CoA reductase activity was 5 to 10 fold higher in non-diabetic animals. The data suggest that insulin acts by increasing the portion of HMG-CoA reductase present in its active free sulfhydryl form.

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Regulation of the activity of 3-hydroxy-3-methylglutaryl coenzyme a reductase by insulin

Abstract

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