Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells

Y. S. Zhu; Y. Lu; G. W. Lin; R. X. Zhang; Z. H. Yang; Y. G. Lu

Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells

Y. S. Zhu, Y. Lu, G. W. Lin, R. X. Zhang, Z. H. Yang, Y. G. Lu

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-X_L, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-X_L in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - X_L between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-X_L play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

Original language	English (US)
Pages (from-to)	32-34
Number of pages	3
Journal	Journal of Shanghai Medical University
Volume	28
Issue number	1
State	Published - 2001

All Science Journal Classification (ASJC) codes

General Medicine

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title = "Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells",

abstract = "Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.",

author = "Zhu, {Y. S.} and Y. Lu and Lin, {G. W.} and Zhang, {R. X.} and Yang, {Z. H.} and Lu, {Y. G.}",

year = "2001",

language = "English (US)",

volume = "28",

pages = "32--34",

journal = "Journal of Shanghai Medical University",

issn = "0257-8131",

publisher = "Fudan University",

number = "1",

}

TY - JOUR

T1 - Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells

AU - Zhu, Y. S.

AU - Lu, Y.

AU - Lin, G. W.

AU - Zhang, R. X.

AU - Yang, Z. H.

AU - Lu, Y. G.

PY - 2001

Y1 - 2001

N2 - Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

AB - Purpose To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins(Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0) %. While the rates in K562 were (1.0 ± 0.3) % and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562(n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

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M3 - Article

AN - SCOPUS:0035112386

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Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells

Abstract

All Science Journal Classification (ASJC) codes

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