Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells

Yu Sheng Zhu; Yuan Lu; Guo Wei Lin; Rong Xing Zhang; Zheng Hong Yang; Yuan Gang Lu

Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells

Yu Sheng Zhu
, Yuan Lu
, Guo Wei Lin
, Rong Xing Zhang
, Zheng Hong Yang
, Yuan Gang Lu

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods: Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-X^L, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results: The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-X_L in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0). While the rates in K562 were (1.0 ± 0.3)% and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - X_L between K562/VCR and K562 (n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions: We suggest that Bcl-2 and Bcl-X_L play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

Original language	English (US)
Pages (from-to)	32-34
Number of pages	3
Journal	Fudan University Journal of Medical Sciences
Volume	28
Issue number	1
State	Published - 2001

All Science Journal Classification (ASJC) codes

General Medicine

Cite this

@article{f5bbba655bf14a6bbc7041d852ba312e,

title = "Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells",

abstract = "Purpose: To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods: Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results: The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) \% and (60.0 ± 10.0). While the rates in K562 were (1.0 ± 0.3)\% and (20.0 ± 4.0)\%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 (n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions: We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.",

author = "Zhu, \{Yu Sheng\} and Yuan Lu and Lin, \{Guo Wei\} and Zhang, \{Rong Xing\} and Yang, \{Zheng Hong\} and Lu, \{Yuan Gang\}",

year = "2001",

language = "English (US)",

volume = "28",

pages = "32--34",

journal = "Fudan University Journal of Medical Sciences",

issn = "1672-8467",

publisher = "Fudan University",

number = "1",

}

TY - JOUR

T1 - Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells

AU - Zhu, Yu Sheng

AU - Lu, Yuan

AU - Lin, Guo Wei

AU - Zhang, Rong Xing

AU - Yang, Zheng Hong

AU - Lu, Yuan Gang

PY - 2001

Y1 - 2001

N2 - Purpose: To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods: Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results: The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0). While the rates in K562 were (1.0 ± 0.3)% and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 (n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions: We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

AB - Purpose: To explore the Relationship between expression of apoptosis-modulating proteins and multidrug resistance in K562/VCR cells. Methods: Immunocytochemical method and western blot were used to analyze the expression of apoptosis-modulating proteins (Bcl - 2, Bcl-XL, Bax, Bak) in multidrug resistant cell line K562/VCR and drugsensitive cell line K562. Results: The positive cell rates of apoptosis-suppressing protein Bcl-2 and Bcl-XL in K562/VCR were (40.0 ± 8.0) % and (60.0 ± 10.0). While the rates in K562 were (1.0 ± 0.3)% and (20.0 ± 4.0)%. There was significant difference in the positive cell rates of Bcl - 2 and Bcl - XL between K562/VCR and K562 (n = 3, P < 0.05). It was also found there was no significant difference in expression of Bax between K562/VCR and K562. Furthermore, Bak was not expressed in both K562/VCR and K562 or the expression was very low. Conclusions: We suggest that Bcl-2 and Bcl-XL play important roles in multidrug resistance in K562/VCR, while Bax and Bak might not be important.

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M3 - Article

AN - SCOPUS:0041315553

SN - 1672-8467

VL - 28

SP - 32

EP - 34

JO - Fudan University Journal of Medical Sciences

JF - Fudan University Journal of Medical Sciences

IS - 1

ER -

Relationship between expression of apoptosis-modulating proteins and multidrug resistance in KS62/VCR cells

Abstract

All Science Journal Classification (ASJC) codes

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