Repair of O-alkylpyrimidines in mammalian cells: A present consensus

T. P. Brent; M. E. Dolan; H. Fraenkel-Conrat; J. Hall; P. Karran; F. Laval; G. P. Margison; R. Montesano; A. E. Pegg; P. M. Potter; B. Singer; J. A. Swenberg; D. B. Yarosh

doi:10.1073/pnas.85.6.1759

Repair of O-alkylpyrimidines in mammalian cells: A present consensus

T. P. Brent, M. E. Dolan, H. Fraenkel-Conrat, J. Hall, P. Karran, F. Laval, G. P. Margison, R. Montesano, A. E. Pegg, P. M. Potter, B. Singer, J. A. Swenberg, D. B. Yarosh

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Abstract

Enzymatic repair of the O-alkylpyrimidines (O²- and O⁴-alkylthymine, O²-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase (DNA-3-methyladenine glycosylase) and a methyltransferase (DNA-O⁶-methylguanine:protein-L-cysteine S-methyltransferase, EC 2.1.1.63), have been well characterized. In mammals or mammalian cells treated with carcinogenic alkylating agents, loss of these derivatives has been demonstrated repeatedly. Nevertheless, mammalian repair proteins that are analogous to those from E. coli do not detectably act on these alkyl derivatives. A variety of techniques has been used by many investigators in the United States and Europe, who conclude here that the mode of O-alkylpyrimidine and alkyl phosphotriester repair in mammalian cells differs from that in E. coli. New approaches and methods are needed to characterize these processes at the biochemical and molecular level.

Original language	English (US)
Pages (from-to)	1759-1762
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	6
DOIs	https://doi.org/10.1073/pnas.85.6.1759
State	Published - 1988

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Brent, T. P., Dolan, M. E., Fraenkel-Conrat, H., Hall, J., Karran, P., Laval, F., Margison, G. P., Montesano, R., Pegg, A. E., Potter, P. M., Singer, B., Swenberg, J. A., & Yarosh, D. B. (1988). Repair of O-alkylpyrimidines in mammalian cells: A present consensus. Proceedings of the National Academy of Sciences of the United States of America, 85(6), 1759-1762. https://doi.org/10.1073/pnas.85.6.1759

@article{7355af1f8c214cfdaa6b501eee97941b,

title = "Repair of O-alkylpyrimidines in mammalian cells: A present consensus",

abstract = "Enzymatic repair of the O-alkylpyrimidines (O2- and O4-alkylthymine, O2-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase (DNA-3-methyladenine glycosylase) and a methyltransferase (DNA-O6-methylguanine:protein-L-cysteine S-methyltransferase, EC 2.1.1.63), have been well characterized. In mammals or mammalian cells treated with carcinogenic alkylating agents, loss of these derivatives has been demonstrated repeatedly. Nevertheless, mammalian repair proteins that are analogous to those from E. coli do not detectably act on these alkyl derivatives. A variety of techniques has been used by many investigators in the United States and Europe, who conclude here that the mode of O-alkylpyrimidine and alkyl phosphotriester repair in mammalian cells differs from that in E. coli. New approaches and methods are needed to characterize these processes at the biochemical and molecular level.",

author = "Brent, {T. P.} and Dolan, {M. E.} and H. Fraenkel-Conrat and J. Hall and P. Karran and F. Laval and Margison, {G. P.} and R. Montesano and Pegg, {A. E.} and Potter, {P. M.} and B. Singer and Swenberg, {J. A.} and Yarosh, {D. B.}",

year = "1988",

doi = "10.1073/pnas.85.6.1759",

language = "English (US)",

volume = "85",

pages = "1759--1762",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "6",

}

Brent, TP, Dolan, ME, Fraenkel-Conrat, H, Hall, J, Karran, P, Laval, F, Margison, GP, Montesano, R, Pegg, AE, Potter, PM, Singer, B, Swenberg, JA & Yarosh, DB 1988, 'Repair of O-alkylpyrimidines in mammalian cells: A present consensus', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 6, pp. 1759-1762. https://doi.org/10.1073/pnas.85.6.1759

TY - JOUR

T1 - Repair of O-alkylpyrimidines in mammalian cells

T2 - A present consensus

AU - Brent, T. P.

AU - Dolan, M. E.

AU - Fraenkel-Conrat, H.

AU - Hall, J.

AU - Karran, P.

AU - Laval, F.

AU - Margison, G. P.

AU - Montesano, R.

AU - Pegg, A. E.

AU - Potter, P. M.

AU - Singer, B.

AU - Swenberg, J. A.

AU - Yarosh, D. B.

PY - 1988

Y1 - 1988

N2 - Enzymatic repair of the O-alkylpyrimidines (O2- and O4-alkylthymine, O2-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase (DNA-3-methyladenine glycosylase) and a methyltransferase (DNA-O6-methylguanine:protein-L-cysteine S-methyltransferase, EC 2.1.1.63), have been well characterized. In mammals or mammalian cells treated with carcinogenic alkylating agents, loss of these derivatives has been demonstrated repeatedly. Nevertheless, mammalian repair proteins that are analogous to those from E. coli do not detectably act on these alkyl derivatives. A variety of techniques has been used by many investigators in the United States and Europe, who conclude here that the mode of O-alkylpyrimidine and alkyl phosphotriester repair in mammalian cells differs from that in E. coli. New approaches and methods are needed to characterize these processes at the biochemical and molecular level.

AB - Enzymatic repair of the O-alkylpyrimidines (O2- and O4-alkylthymine, O2-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase (DNA-3-methyladenine glycosylase) and a methyltransferase (DNA-O6-methylguanine:protein-L-cysteine S-methyltransferase, EC 2.1.1.63), have been well characterized. In mammals or mammalian cells treated with carcinogenic alkylating agents, loss of these derivatives has been demonstrated repeatedly. Nevertheless, mammalian repair proteins that are analogous to those from E. coli do not detectably act on these alkyl derivatives. A variety of techniques has been used by many investigators in the United States and Europe, who conclude here that the mode of O-alkylpyrimidine and alkyl phosphotriester repair in mammalian cells differs from that in E. coli. New approaches and methods are needed to characterize these processes at the biochemical and molecular level.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Repair of O-alkylpyrimidines in mammalian cells: A present consensus

Abstract

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