TY - JOUR
T1 - Replication and recombination functions associated with the yeast plasmid, 2μ circle
AU - Broach, James R.
AU - Hicks, James B.
N1 - Funding Information:
We would like to thank Dr. J. Strathern for helpful discussions during the course of this work, Drs. C. Newlon and J. Hartley for communicating unpublished results, Ms. V. Guarascio and Ms. C. McGill for technical assistance and Ms. L. Dalessandro for preparation of the manuscript. This work was supported in part by NIH grants to J.R.B. and J.B.H.
PY - 1980
Y1 - 1980
N2 - By examining both the transformation efficiency of yeast of various plasmids containing defined regions of the 2μ circle genome and the characteristics of the resultant transformants, we have identified several regions of the 2μ circle genome which are involved in 2μ circle replication or recombination. First, by identifying those DNA fragments from the molecule which promote high frequency transformation of yeast, we have localized the origin of replication to a sequence partially within the large unique region, which, as determined by subsequent deletion analysis, extends from the middle of the inverted repeat region into the contiguous unique region. Second, by examining the relative efficiency of replication in yeast of hybrid plasmids containing either the entire 2μ circle genome or a fragment of 2μ circle encompassing the origin of replication, we have determined that efficient use of the 2μ circle origin requires some function or functions encoded in the molecule at a site away from the origin. Third, by examining the ability of a mutant 2μ circle molecule to undergo intramolecular recombination in yeast, we have identified a 2μ circle gene which codes for a product required for this process.
AB - By examining both the transformation efficiency of yeast of various plasmids containing defined regions of the 2μ circle genome and the characteristics of the resultant transformants, we have identified several regions of the 2μ circle genome which are involved in 2μ circle replication or recombination. First, by identifying those DNA fragments from the molecule which promote high frequency transformation of yeast, we have localized the origin of replication to a sequence partially within the large unique region, which, as determined by subsequent deletion analysis, extends from the middle of the inverted repeat region into the contiguous unique region. Second, by examining the relative efficiency of replication in yeast of hybrid plasmids containing either the entire 2μ circle genome or a fragment of 2μ circle encompassing the origin of replication, we have determined that efficient use of the 2μ circle origin requires some function or functions encoded in the molecule at a site away from the origin. Third, by examining the ability of a mutant 2μ circle molecule to undergo intramolecular recombination in yeast, we have identified a 2μ circle gene which codes for a product required for this process.
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U2 - 10.1016/0092-8674(80)90487-0
DO - 10.1016/0092-8674(80)90487-0
M3 - Article
C2 - 7407923
AN - SCOPUS:0018955095
SN - 0092-8674
VL - 21
SP - 501
EP - 508
JO - Cell
JF - Cell
IS - 2
ER -