TY - JOUR
T1 - Reproduction and metabolism at -10°C of bacteria isolated from Siberian permafrost
AU - Bakermans, Corlen
AU - Tsapin, Alexandre I.
AU - Souza-Egipsy, Virginia
AU - Gilichinsky, David A.
AU - Nealson, Kenneth H.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - We report the isolation and properties of several species of bacteria from Siberian permafrost. Half of the isolates were spore-forming bacteria unable to grow or metabolize at subzero temperatures. Other Gram-positive isolates metabolized, but never exhibited any growth at -10°C. One Gram-negative isolate metabolized and grew at -10°C, with a measured doubling time of 39 days. Metabolic studies of several isolates suggested that as temperature decreased below +4°C, the partitioning of energy changes with much more energy being used for cell maintenance as the temperature decreases. In addition, cells grown at -10°C exhibited major morphological changes at the ultrastructural level.
AB - We report the isolation and properties of several species of bacteria from Siberian permafrost. Half of the isolates were spore-forming bacteria unable to grow or metabolize at subzero temperatures. Other Gram-positive isolates metabolized, but never exhibited any growth at -10°C. One Gram-negative isolate metabolized and grew at -10°C, with a measured doubling time of 39 days. Metabolic studies of several isolates suggested that as temperature decreased below +4°C, the partitioning of energy changes with much more energy being used for cell maintenance as the temperature decreases. In addition, cells grown at -10°C exhibited major morphological changes at the ultrastructural level.
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U2 - 10.1046/j.1462-2920.2003.00419.x
DO - 10.1046/j.1462-2920.2003.00419.x
M3 - Article
C2 - 12662179
AN - SCOPUS:0242500335
SN - 1462-2912
VL - 5
SP - 321
EP - 326
JO - Environmental microbiology
JF - Environmental microbiology
IS - 4
ER -