TY - JOUR
T1 - Requirement of a dynein light chain in transforming growth factor β signaling in zebrafish ovarian follicle cells
AU - Jin, Qunyan
AU - Gao, Guofeng
AU - Mulder, Kathleen M.
N1 - Funding Information:
This work was supported by National Institutes of Health Grants CA100239 , CA090765 , CA092889 and CA092889-08S1 , as well as Department of Defense award DAMD 17-03-1-0287, to K.M.M. We thank Dr. J. Massague (Memorial Sloan-Kettering Cancer Center, New York, NY) for the 3TP-Lux and KNRII-HA constructs and DR26 cells. We also thank Dr. H. Moses (Vanderbilt University) for the RI-T204D-HA, Dr. Malcolm Whitman (Harvard Medical School, Boston, MA) for the ARE-Lux and FAST-1 constructs, and Dr. J. Wrana (Samuel Lundenfeld Res. Institute, Toronto, Canada) for pCMV5-HA-RII.
PY - 2012/1/2
Y1 - 2012/1/2
N2 - We have previously reported that the dynein light chains km23-1 and km23-2 are required for TGFβ signaling in mammalian cells. Here we describe another member of the km23/DYNLRB/LC7/robl family of dynein light chains in zebrafish, termed zkm23, which is also involved in TGFβ signaling. zkm23 was rapidly phosphorylated after TGFβ stimulation. TGFβ RII kinase activity was absolutely required for zkm23 phosphorylation, whereas a constitutively active TGFβ RI did not induce phosphorylation. Further, TGFβ stimulated a rapid recruitment of the zkm23 dynein light chain to the dynein intermediate chain of the dynein complex, and the TGFβ RII kinase was required for this interaction. Finally, blockade of zkm23 using morpholino oligos resulted in an inhibition of TGFβ-mediated transcriptional responses. Thus, our results demonstrate for the first time that the dynein light chain zkm23 is required for TGFβ signaling in cultured zebrafish ovarian follicle cells.
AB - We have previously reported that the dynein light chains km23-1 and km23-2 are required for TGFβ signaling in mammalian cells. Here we describe another member of the km23/DYNLRB/LC7/robl family of dynein light chains in zebrafish, termed zkm23, which is also involved in TGFβ signaling. zkm23 was rapidly phosphorylated after TGFβ stimulation. TGFβ RII kinase activity was absolutely required for zkm23 phosphorylation, whereas a constitutively active TGFβ RI did not induce phosphorylation. Further, TGFβ stimulated a rapid recruitment of the zkm23 dynein light chain to the dynein intermediate chain of the dynein complex, and the TGFβ RII kinase was required for this interaction. Finally, blockade of zkm23 using morpholino oligos resulted in an inhibition of TGFβ-mediated transcriptional responses. Thus, our results demonstrate for the first time that the dynein light chain zkm23 is required for TGFβ signaling in cultured zebrafish ovarian follicle cells.
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U2 - 10.1016/j.mce.2011.08.029
DO - 10.1016/j.mce.2011.08.029
M3 - Article
C2 - 21920407
AN - SCOPUS:80755153644
SN - 0303-7207
VL - 348
SP - 233
EP - 240
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1
ER -