TY - JOUR
T1 - Resolution of myocardial myosin light chains by two dimensional gel electrophoresis
AU - McPherson, J.
AU - Traut, R. R.
AU - Mason, D. T.
AU - Zelis, R.
AU - Wikman-Coffelt, J.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 1974
Y1 - 1974
N2 - Two dimensional gel electrophoresis of canine myocardial light chains of myosin demonstrated the existence of a single polypeptide species corresponding to the lighter component, C2. However, contrary to previous observations, it was found that the heavier polypeptide, C1, although homogeneous by one dimensional electrophoresis in polyacrylamide gels containing dodecyl sulfate, is resolved into 4 components by two dimensional gel electrophoresis at pH 8.7 and 4.5. Of these 4 components 2, named C1c and C1d, present in roughly equal amounts, comprise approximately 95% of the total C1 fraction, while the remaining 5% of the C1 material consists of 2 components, named C1a and C1b.
AB - Two dimensional gel electrophoresis of canine myocardial light chains of myosin demonstrated the existence of a single polypeptide species corresponding to the lighter component, C2. However, contrary to previous observations, it was found that the heavier polypeptide, C1, although homogeneous by one dimensional electrophoresis in polyacrylamide gels containing dodecyl sulfate, is resolved into 4 components by two dimensional gel electrophoresis at pH 8.7 and 4.5. Of these 4 components 2, named C1c and C1d, present in roughly equal amounts, comprise approximately 95% of the total C1 fraction, while the remaining 5% of the C1 material consists of 2 components, named C1a and C1b.
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M3 - Article
C2 - 4811908
AN - SCOPUS:0015949472
SN - 0042-1215
VL - 249
SP - 994
EP - 996
JO - Unknown Journal
JF - Unknown Journal
IS - 3
ER -