Restriction endonuclease cleavage map of the DNA of JC virus

J. D. Martin; R. J. Frisque; B. L. Padgett; D. L. Walker

doi:10.1128/jvi.29.3.846-855.1979

Restriction endonuclease cleavage map of the DNA of JC virus

J. D. Martin, R. J. Frisque, B. L. Padgett, D. L. Walker

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A physical map of the sites cleaved by the following restriction endonucleases was derived for the DNA of JC virus, a human polyomavirus: EcoRI, HpaI, and PstI (one site each); HindII (four sites); and HindIII (three sites). By agarose gel electrophoresis of fragmented DNA, the size of full-length DNA of JC virus was estimated to be 5,125 ± 105 base pairs (98 ± 2% of the length of simian virus 40 DNA).

Original language	English (US)
Pages (from-to)	846-855
Number of pages	10
Journal	Journal of virology
Volume	29
Issue number	3
DOIs	https://doi.org/10.1128/jvi.29.3.846-855.1979
State	Published - 1979

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Insect Science
Virology

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10.1128/jvi.29.3.846-855.1979

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AU - Frisque, R. J.

AU - Padgett, B. L.

AU - Walker, D. L.

PY - 1979

Y1 - 1979

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AB - A physical map of the sites cleaved by the following restriction endonucleases was derived for the DNA of JC virus, a human polyomavirus: EcoRI, HpaI, and PstI (one site each); HindII (four sites); and HindIII (three sites). By agarose gel electrophoresis of fragmented DNA, the size of full-length DNA of JC virus was estimated to be 5,125 ± 105 base pairs (98 ± 2% of the length of simian virus 40 DNA).

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Restriction endonuclease cleavage map of the DNA of JC virus

Abstract

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