Restriction fragment length polymorphism of c4 genes in mice with t chromosomes

Mladen Golubić, Felipe Figueroa, Mario Tosi, Jan Klein

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12 Scopus citations


Genomic DNA was isolated from 29 t strains and 4 congenic lines of mice, digested with restriction endonucleases, and hybridized with a probe representing the complement component 4 (C4) gene. All but one of the enzymes revealed restriction fragment length polymorphism in this sample of C4-related genes. Double digestion analysis suggested the presence of three C4 gene copies in some of the t chromosomes and two copies in others. The enzymes distinguished 16 different haplotypes among the 33 strains tested. Based on their restriction fragment length patterns, the t strains could be divided into four groups with strains in each group more closely related to each other with respect to their C4-region genes than strains belonging to different groups. At least three of these four groups represent different branches of the evolutionary tree constructed for the t chromosomes. The C4-related genes of the chromosomes are in strong linkage disequilibrium with the class II genes of the H-2 complex. Typing for the Ss and Slp allotypes of C4 has revealed the presence of the Ss1 phenotype in two t strains and of the Slpa phenotype in one strain.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
Issue number3
StatePublished - Mar 1985

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics


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