Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine

Terry J. Smith; James T. Warren; Ellis L. Kline

doi:10.1016/0006-291X(89)91994-3

Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine

Terry J. Smith, James T. Warren, Ellis L. Kline

School of Science (Behrend)

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The effect of retinoic acid on the induction of tyrosinase (EC 1:14.18.1) by imidazole was determined in cultured B16 C3 melanoma cells. Retinoic acid could block the induction of enzyme activity within 3 hours of addition to the culture medium at a physiological concentration (10nM). The blockade was similar to that of 3,3′,5-L-triiodothyronine (T₃) already reported. mRNA hybridizable to a tyrosinase DNA probe was induced by imidazole while retinoic acid and T₃ blocked that increase. These observations suggest that retinoic acid can mimic the action of T₃ in B16 melanoma cells in culture.

Original language	English (US)
Pages (from-to)	288-293
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	162
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(89)91994-3
State	Published - Jul 14 1989

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(89)91994-3

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title = "Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine",

abstract = "The effect of retinoic acid on the induction of tyrosinase (EC 1:14.18.1) by imidazole was determined in cultured B16 C3 melanoma cells. Retinoic acid could block the induction of enzyme activity within 3 hours of addition to the culture medium at a physiological concentration (10nM). The blockade was similar to that of 3,3′,5-L-triiodothyronine (T3) already reported. mRNA hybridizable to a tyrosinase DNA probe was induced by imidazole while retinoic acid and T3 blocked that increase. These observations suggest that retinoic acid can mimic the action of T3 in B16 melanoma cells in culture.",

author = "Smith, {Terry J.} and Warren, {James T.} and Kline, {Ellis L.}",

note = "Funding Information: ACKNOWLEDGMENTS: The authors wish to thank Mrs. Theresa Kluge and Carina Calovini for preparing the manuscript. The work was supported in part by the Research Service of the Veterans Administration and by the ELK-PAM research fund. Address all correspondence to Terry J.Smith, M.D., Veterans Administration Medical Center, 3495 Bailey Avenue, Buffalo, NY 14215.",

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language = "English (US)",

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Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine. / Smith, Terry J.; Warren, James T.; Kline, Ellis L.
In: Biochemical and Biophysical Research Communications, Vol. 162, No. 1, 14.07.1989, p. 288-293.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures

T2 - Similar effects of the active retinoid and triiodothyronine

AU - Smith, Terry J.

AU - Warren, James T.

AU - Kline, Ellis L.

N1 - Funding Information: ACKNOWLEDGMENTS: The authors wish to thank Mrs. Theresa Kluge and Carina Calovini for preparing the manuscript. The work was supported in part by the Research Service of the Veterans Administration and by the ELK-PAM research fund. Address all correspondence to Terry J.Smith, M.D., Veterans Administration Medical Center, 3495 Bailey Avenue, Buffalo, NY 14215.

PY - 1989/7/14

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N2 - The effect of retinoic acid on the induction of tyrosinase (EC 1:14.18.1) by imidazole was determined in cultured B16 C3 melanoma cells. Retinoic acid could block the induction of enzyme activity within 3 hours of addition to the culture medium at a physiological concentration (10nM). The blockade was similar to that of 3,3′,5-L-triiodothyronine (T3) already reported. mRNA hybridizable to a tyrosinase DNA probe was induced by imidazole while retinoic acid and T3 blocked that increase. These observations suggest that retinoic acid can mimic the action of T3 in B16 melanoma cells in culture.

AB - The effect of retinoic acid on the induction of tyrosinase (EC 1:14.18.1) by imidazole was determined in cultured B16 C3 melanoma cells. Retinoic acid could block the induction of enzyme activity within 3 hours of addition to the culture medium at a physiological concentration (10nM). The blockade was similar to that of 3,3′,5-L-triiodothyronine (T3) already reported. mRNA hybridizable to a tyrosinase DNA probe was induced by imidazole while retinoic acid and T3 blocked that increase. These observations suggest that retinoic acid can mimic the action of T3 in B16 melanoma cells in culture.

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Retinoic acid blockade of imidazole-induced tyrosinase expression in B16 melanoma cultures: Similar effects of the active retinoid and triiodothyronine

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