TY - JOUR
T1 - Retinoic acid promotes mouse splenic B cell surface IgG expression and maturation stimulated by CD40 and IL-4
AU - Chen, Qiuyan
AU - Ross, A. Catharine
N1 - Funding Information:
We thank Elaine Kunze and the staff of the Center for Quantitative Cell Analysis, Penn State, for their advice and assistance with flow analysis and cell sorting. Financial support was provided by NIH Grant DK-41479 and funds from the Dorothy Foehr Huck Chair.
PY - 2007/9
Y1 - 2007/9
N2 - Retinoic acid (RA) increases antibody production in vivo but its role in B-cell activation is unclear. In a model of purified mouse splenic B cells stimulated by CD40 coreceptor (as a surrogate of T cell co-stimulation), IL-4, a principal Th-2 cytokine, and ligation of the B-cell antigen receptor, CD40 engagement or IL-4 alone induced B-cell activation indicated by increased Igγ1 germline transcripts, cell proliferation, and surface (s)IgG1 expression, while triple stimulation with the combination of anti-CD40/IL-4/anti-μ synergized to heighten B-cell activation. Although RA was growth inhibitory for anti-CD40-activated B cells, RA increased the proportion of B cells that had more differentiated phenotypes, such as expression of higher level of activation-induced deaminase, Blimp-1, CD138/syndecan-1 and sIgG1. Overall, RA can promote B-cell maturation at the population level by increasing the number of sIgG1 and CD138 expressing cells, which may be related to the potentiation of humoral immunity in vivo.
AB - Retinoic acid (RA) increases antibody production in vivo but its role in B-cell activation is unclear. In a model of purified mouse splenic B cells stimulated by CD40 coreceptor (as a surrogate of T cell co-stimulation), IL-4, a principal Th-2 cytokine, and ligation of the B-cell antigen receptor, CD40 engagement or IL-4 alone induced B-cell activation indicated by increased Igγ1 germline transcripts, cell proliferation, and surface (s)IgG1 expression, while triple stimulation with the combination of anti-CD40/IL-4/anti-μ synergized to heighten B-cell activation. Although RA was growth inhibitory for anti-CD40-activated B cells, RA increased the proportion of B cells that had more differentiated phenotypes, such as expression of higher level of activation-induced deaminase, Blimp-1, CD138/syndecan-1 and sIgG1. Overall, RA can promote B-cell maturation at the population level by increasing the number of sIgG1 and CD138 expressing cells, which may be related to the potentiation of humoral immunity in vivo.
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U2 - 10.1016/j.cellimm.2007.11.001
DO - 10.1016/j.cellimm.2007.11.001
M3 - Article
C2 - 18082674
AN - SCOPUS:38349194826
SN - 0008-8749
VL - 249
SP - 37
EP - 45
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -