Retracing enzyme evolution in the (βα)8-barrel scaffold

James D. Stevenson; Stefan Lutz; Stephen Benkovic

doi:10.1002/1521-3773(20010518)40:10<1854::AID-ANIE1854>3.0.CO;2-F

Retracing enzyme evolution in the (βα)₈-barrel scaffold

James D. Stevenson, Stefan Lutz, Stephen Benkovic

Chemistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

"Refitting" existing protein scaffolds to generate new enzymes with designed properties is a powerful tool in molecular evolution. Two recently published reports demonstrate the engineering of the ubiquitous (βα)₈-Barrel (see picture) scaffold to obtain new enzymatic catalytic properties. These are the first examples of the conversion of the enzyme's activity into that of another by using directed evaluation.

Original language	English (US)
Pages (from-to)	1854-1856
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	40
Issue number	10
DOIs	https://doi.org/10.1002/1521-3773(20010518)40:10<1854::AID-ANIE1854>3.0.CO;2-F
State	Published - May 18 2001

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

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10.1002/1521-3773(20010518)40:10<1854::AID-ANIE1854>3.0.CO;2-F

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AB - "Refitting" existing protein scaffolds to generate new enzymes with designed properties is a powerful tool in molecular evolution. Two recently published reports demonstrate the engineering of the ubiquitous (βα)8-Barrel (see picture) scaffold to obtain new enzymatic catalytic properties. These are the first examples of the conversion of the enzyme's activity into that of another by using directed evaluation.

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Retracing enzyme evolution in the (βα)₈-barrel scaffold

Abstract

All Science Journal Classification (ASJC) codes

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