Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

C. W. Ronson, B. T. Nixon, L. M. Albright, F. M. Ausubel

Research output: Contribution to journalArticlepeer-review

145 Scopus citations


We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

Original languageEnglish (US)
Pages (from-to)2424-2431
Number of pages8
JournalJournal of bacteriology
Issue number6
StatePublished - 1987

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology


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