Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

C. W. Ronson; B. T. Nixon; L. M. Albright; F. M. Ausubel

doi:10.1128/jb.169.6.2424-2431.1987

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

C. W. Ronson
, B. T. Nixon
, L. M. Albright
, F. M. Ausubel

Plant Institute

Research output: Contribution to journal › Article › peer-review

145 Scopus citations

Abstract

We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C₄-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

Original language	English (US)
Pages (from-to)	2424-2431
Number of pages	8
Journal	Journal of bacteriology
Volume	169
Issue number	6
DOIs	https://doi.org/10.1128/jb.169.6.2424-2431.1987
State	Published - 1987

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology

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10.1128/jb.169.6.2424-2431.1987

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title = "Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions",

abstract = "We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38\% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.",

author = "Ronson, \{C. W.\} and Nixon, \{B. T.\} and Albright, \{L. M.\} and Ausubel, \{F. M.\}",

year = "1987",

doi = "10.1128/jb.169.6.2424-2431.1987",

language = "English (US)",

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pages = "2424--2431",

journal = "Journal of bacteriology",

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T1 - Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

AU - Ronson, C. W.

AU - Nixon, B. T.

AU - Albright, L. M.

AU - Ausubel, F. M.

PY - 1987

Y1 - 1987

N2 - We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

AB - We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.

UR - https://www.scopus.com/pages/publications/0023262822

UR - https://www.scopus.com/pages/publications/0023262822#tab=citedBy

U2 - 10.1128/jb.169.6.2424-2431.1987

DO - 10.1128/jb.169.6.2424-2431.1987

M3 - Article

C2 - 3034856

AN - SCOPUS:0023262822

SN - 0021-9193

VL - 169

SP - 2424

EP - 2431

JO - Journal of bacteriology

JF - Journal of bacteriology

IS - 6

ER -

Rhizobium meliloti ntrA (rpoN) gene is required for diverse metabolic functions

Abstract

All Science Journal Classification (ASJC) codes

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